ArticleBiophysical journal2008
Voltage-dependent C-type inactivation in a constitutively open K+ channel.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Mechanism of external K+ sensitivity of KCNQ1 channels.The Journal of general physiology · 2023Article
- KCNQ Potassium Channels as Targets of Botanical Folk Medicines.Annual review of pharmacology and toxicology · 2022Review
- Electrophysiological Properties from Computations at a Single Voltage: Testing Theory with Stochastic Simulations.Entropy (Basel, Switzerland) · 2021Article
- The ubiquitous flavonoid quercetin is an atypical KCNQ potassium channel activator.Communications biology · 2020Article
- Synaptic cleft microenvironment influences potassium permeation and synaptic transmission in hair cells surrounded by calyx afferents in the turtle.The Journal of physiology · 2020Article
- KCNQs: Ligand- and Voltage-Gated Potassium Channels.Frontiers in physiology · 2020Review
- Chansporter complexes in cell signaling.FEBS letters · 2017Review
- Identification of Intrahelical Bifurcated H-Bonds as a New Type of Gate in KJournal of the American Chemical Society · 2017Article
- Grafting voltage and pharmacological sensitivity in potassium channels.Cell research · 2016Article
- Closed state-coupled C-type inactivation in BK channels.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Review
- KCNE genetics and pharmacogenomics in cardiac arrhythmias: much ado about nothing?Expert review of clinical pharmacology · 2013Review
- High-risk long QT syndrome mutations in the Kv7.1 (KCNQ1) pore disrupt the molecular basis for rapid K(+) permeation.Biochemistry · 2012Article
- Pore helix-S6 interactions are critical in governing current amplitudes of KCNQ3 K+ channels.Biophysical journal · 2012Article
- Structural correlates of selectivity and inactivation in potassium channels.Biochimica et biophysica acta · 2012Review
- Regions of KCNQ K(+) channels controlling functional expression.Frontiers in physiology · 2012Article
- Allosteric features of KCNQ1 gating revealed by alanine scanning mutagenesis.Biophysical journal · 2011Article
- The S4-S5 linker of KCNQ1 channels forms a structural scaffold with the S6 segment controlling gate closure.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Most voltage-gated potassium (Kv) channels undergo C-type inactivation during sustained depolarization. The voltage dependence and other mechanistic aspects of this process are debated, and difficult to elucidate because of concomitant voltage-dependent activation. Here, we demonstrate that MinK-KCNQ1 (I(Ks)) channels with an S6-domain mutation, F340W in KCNQ1, exhibit constitutive activation but voltage-dependent C-type inactivation. F340W-I(Ks) inactivation was sensitive to extracellular cation concentration and species, and it altered ion selectivity, suggestive of pore constriction. The rate and extent of F340W-I(Ks) inactivation and recovery from inactivation were voltage-dependent with physiologic intracellular ion concentrations, and in the absence or presence of external K(+), with an estimated gating charge, z(i), of approximately 1. Finally, double-mutant channels with a single S4 charge neutralization (R231A,F340W-I(Ks)) exhibited constitutive C-type inactivation. The results suggest that F340W-I(Ks) channels exhibit voltage-dependent C-type inactivation involving S4, without the necessity for voltage-dependent opening, allosteric coupling to voltage-dependent S6 transitions occurring during channel opening, or voltage-dependent changes in ion occupancy. The data also identify F340 as a critical hub for KCNQ1 gating processes and their modulation by MinK, and present a unique system for further mechanistic studies of the role of coupling of C-type inactivation to S4 movement, without contamination from voltage-dependent activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.