ArticleThe Journal of biological chemistry2008
Membrane composition modulates prestin-associated charge movement.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- From transporter to motor: Evolutionary and structural insights into the emergence of prestin's area-motor activity in mammals.Protein science : a publication of the Protein Society · 2026Article
- MYH1 deficiency disrupts outer hair cell electromotility, resulting in hearing loss.Experimental & molecular medicine · 2024Article
- LHFPL5 is a key element in force transmission from the tip link to the hair cell mechanotransducer channel.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Folding of prestin's anion-binding site and the mechanism of outer hair cell electromotility.eLife · 2023Article
- Phytosterols reverse antiretroviral-induced hearing loss, with potential implications for cochlear aging.PLoS biology · 2023Article
- Coupling between outer hair cell electromotility and prestin sensor charge depends on voltage operating point.Hearing research · 2022Article
- Single particle cryo-EM structure of the outer hair cell motor protein prestin.Nature communications · 2022Article
- Article
- State dependent effects on the frequency response of prestin's real and imaginary components of nonlinear capacitance.Scientific reports · 2021Article
- A novel theoretical framework reveals more than one voltage-sensing pathway in the lateral membrane of outer hair cells.The Journal of general physiology · 2020Article
- Diflunisal inhibits prestin by chloride-dependent mechanism.PloS one · 2017Article
- Membrane prestin expression correlates with the magnitude of prestin-associated charge movement.Hearing research · 2016Article
- Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent.Scientific reports · 2016Article
- Localization and proteomic characterization of cholesterol-rich membrane microdomains in the inner ear.Journal of proteomics · 2014Article
- Effects of cholesterol alterations are mediated via G-protein-related pathways in outer hair cells.Pflugers Archiv : European journal of physiology · 2013Article
- Microdomains shift and rotate in the lateral wall of cochlear outer hair cells.Biophysical journal · 2013Article
- Prestin regulation and function in residual outer hair cells after noise-induced hearing loss.PloS one · 2013Article
- Effects of plasma membrane cholesterol level and cytoskeleton F-actin on cell protrusion mechanics.PloS one · 2013Article
- Membrane cholesterol strongly influences confined diffusion of prestin.Biophysical journal · 2012Article
- Effects of cholesterol on nano-mechanical properties of the living cell plasma membrane.Soft matter · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The lateral membrane of the cochlear outer hair cell (OHC) is the site of a membrane-based motor that powers OHC electromotility, enabling amplification and fine-tuning of auditory signals. The OHC membrane protein prestin plays a central role in this process. We have previously shown that membrane cholesterol modulates the peak voltage of prestin-associated nonlinear capacitance in vivo and in vitro. The present study explores the effects of membrane cholesterol and docosahexaenoic acid content on the peak and magnitude of prestin-associated charge movement in a human embryonic kidney (HEK 293) cell model. Increasing membrane cholesterol results in a hyperpolarizing shift in the peak voltage of the nonlinear capacitance (Vpkc) and a decrease in the total charge movement. Both measures depend linearly on membrane cholesterol concentration. Incubation of cholesterol-loaded cells in cholesterol-free media partially restores the Vpkc toward normal values but does not have a compensatory effect on the total charge movement. Decreasing membrane cholesterol results in a depolarizing shift in Vpkc that is restored toward normal values upon incubation in cholesterol-free media. However, cholesterol depletion does not alter the magnitude of charge movement. In contrast, increasing membrane docosahexaenoic acid results in a hyperpolarizing shift in Vpkc that is accompanied by an increase in total charge movement. Our results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.