ArticleOsteoarthritis and cartilage2008
Exuberant expression of chemokine genes by adult human articular chondrocytes in response to IL-1beta.
Article in Osteoarthritis and cartilage, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 149 citations in OpenAlex.
- Trial
- Effects of CD14 macrophages and proinflammatory cytokines on chondrogenesis in osteoarthritic synovium-derived stem cells.Tissue engineering. Part A · 2014Trial
- Inflammatory synovial fluid microenvironment drives primary human chondrocytes to actively take part in inflammatory joint diseases.Immunologic research · 2012Trial
- Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research.iScience · 2026Article
- A kinetic-based framework for the selection of in vitro osteoarthritis models: integrating temporal dynamics of inflammatory inducers.Molecular biology reports · 2026Review
- Uterine epithelial estrogen receptor α temporally regulates preimplantation uterine immunity involving interleukin-1β signaling.Reproductive and developmental medicine · 2026Article
- A damaging mutation in COL6A3 alters the mechanobiologic response of chondrocytes derived from human induced pluripotent stem cells.Stem cell research & therapy · 2026Article
- RIPK3 is spatially associated with cartilage degeneration in osteoarthritis: integrative transcriptomic and histological analysis.Journal of orthopaedic surgery and research · 2025Article
- Effects of intra-articular applied rat BMSCs expressing alpha-calcitonin gene-related peptide or substance P on osteoarthritis pathogenesis in a murine surgical osteoarthritis model.Stem cell research & therapy · 2025Article
- Mode of injury and level of synovitis alter inflammatory chondrocyte gene expression and associated pathways.Scientific reports · 2024Article
- Micro-current stimulation could inhibit IL-1β-induced inflammatory responses in chondrocytes and protect knee bone cartilage from osteoarthritis.Biomedical engineering letters · 2024Article
- Omega-3 Supplementation and Its Effects on Osteoarthritis.Nutrients · 2024Review
- Adipose-derived regenerative therapies for the treatment of knee osteoarthritis.World journal of stem cells · 2024Article
- CC chemokines and receptors in osteoarthritis: new insights and potential targets.Arthritis research & therapy · 2023Review
- Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in anJournal of orthopaedic translation · 2023Article
- Review
- Inflammation-Driven Secretion Potential Is Upregulated in Osteoarthritic Fibroblast-Like Synoviocytes.International journal of molecular sciences · 2022Article
- Article
- Cytokines and Chemokines Involved in Osteoarthritis Pathogenesis.International journal of molecular sciences · 2021Review
- Inflammatory responses to metal oxide ceramic nanopowders.Scientific reports · 2021Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo provide a more complete picture of the effect of interleukin-1 beta (IL-1beta) on adult human articular chondrocyte gene expression, in contrast to the candidate gene approach.
designChondrocytes from human knee cartilage were cultured in medium containing IL-1beta. Changes in gene expression were analyzed by microarray and reverse transcriptase-polymerase chain reaction analysis. The ability of transforming growth factor beta-1 (TGF-beta1), fibroblast growth factor (FGF)-18, and bone morphogenetic protein 2 (BMP-2) to alter the effects of IL-1beta was analyzed. Computational analysis of the promoter regions of differentially expressed genes for transcription factor binding motifs was performed.
resultsIL-1beta-treated human chondrocytes showed significant increases in the expression of granulocyte colony stimulating factor-3, endothelial leukocyte adhesion molecule 1 and leukemia inhibitory factor as well as for a large group of chemokines that include CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL8, CCL2, CCL3, CCL4, CCL5, CCL8, CCL20, CCL3L1, CX3CL1 and the cytokine IL-6. As expected, the mRNA for matrix metalloproteinase (MMP)-13 and BMP-2 also increased while mRNA for the matrix genes COL2A1 and aggrecan was down-regulated. A subset of chemokines increased rapidly at very low levels of IL-1beta. The phenotype induced by IL-1beta was partially reversed by TGF-beta1, but not by BMP-2. In the presence of IL-1beta, FGF-18 increased expression of ADAMTS-4, aggrecan, BMP-2, COL2A1, CCL3, CCL4, CCL20, CXCL1, CXCL3, CXCL6, IL-1beta, IL-6, and IL-8 and decreased ADAMTS-5, MMP-13, CCL2, and CCL8. Computational analysis revealed a high likelihood that the most up-regulated chemokines are regulated by the transcription factors myocyte enhancer binding factor-3 (MEF-3), CCAAT/enhancer binding protein (C/EBP) and nuclear factor-kappa B (NF-kappaB).
conclusionIL-1beta has a diverse effect on gene expression profile in human chondrocytes affecting matrix genes as well as chemokines and cytokines. TGF-beta1 has the ability to antagonize some of the phenotype induced by IL-1beta.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.