ArticleJournal of immunological methods2008
Polychromatic flow cytometric high-throughput assay to analyze the innate immune response to Toll-like receptor stimulation.
Article in Journal of immunological methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02687503 (Impact of Probiotic Use on Immune Cell Function in Children), which is not on this map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Impact of Probiotic Use on Immune Cell Function in Children
Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Modeling neonatal immune response to B. pertussis identifies early B cell activation and differentiation.PLoS pathogens · 2026Article
- Patients Hospitalized with COVID-19 Demonstrate Distinct Plasma Cytokine and Chemokine Concentrations in vivo and TLR-Mediated Cytokine and Chemokine Production in Whole Blood in vitro.Journal of innate immunity · 2025Article
- Immune profiling reveals umbilical cord blood mononuclear cells from South India display an IL-8 dominant, CXCL-10 deficient polyfunctional monocyte response to pathogen-associated molecular patterns that is distinct from adult blood cells.Clinical and experimental immunology · 2024Article
- Cord blood sphingolipids are associated with atopic dermatitis and wheeze in the first year of life.The journal of allergy and clinical immunology. Global · 2022Article
- Transcriptomic profile investigations highlight a putative role for NUDT16 in sepsis.Journal of cellular and molecular medicine · 2022Article
- Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis.Biology · 2021Article
- Respiratory health, allergies, and the farm environment: design, methods and enrollment in the observational Wisconsin Infant Study Cohort (WISC): a research proposal.BMC research notes · 2019Article
- Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry.Journal of visualized experiments : JoVE · 2018Article
- TLR agonist combinations that stimulate Th type I polarizing responses from human neonates.Innate immunity · 2018Article
- Diagnostic Tools for Inborn Errors of Human Immunity (Primary Immunodeficiencies and Immune Dysregulatory Diseases).Current allergy and asthma reports · 2018Review
- Article
- Functional and Phenotypic Changes of Natural Killer Cells in Whole Blood duringFrontiers in immunology · 2018Article
- All on "CHIP": using microfluidics to study neutrophil ontogeny.Translational research : the journal of laboratory and clinical medicine · 2017Article
- Mass cytometry identifies a distinct monocyte cytokine signature shared by clinically heterogeneous pediatric SLE patients.Journal of autoimmunity · 2017Article
- Lack of broad functional differences in immunity in fully vaccinated vs. unvaccinated children.Pediatric research · 2017Article
- Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased B cell activation.PloS one · 2017Article
- Immune cell dysfunctions in breast cancer patients detected through whole blood multi-parametric flow cytometry assay.Oncoimmunology · 2016Article
- Attenuated innate immune defenses in very premature neonates during the neonatal period.Pediatric research · 2015Article
- Single-cell systems-level analysis of human Toll-like receptor activation defines a chemokine signature in patients with systemic lupus erythematosus.The Journal of allergy and clinical immunology · 2015Article
- Single-cell analysis of innate cytokine responses to pattern recognition receptor stimulation in children across four continents.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Polychromatic flow cytometry allows the capture of multidimensional data, providing the technical tool to assess complex immune responses. Interrogation of the adaptive T cell response to infection or vaccination already has benefited greatly from standardized protocols for polychromatic flow cytometric analysis. The innate immune system plays an important role in health and disease, and presents potentially important therapeutic and diagnostic modalities. We describe here a high-throughput polychromatic flow cytometry-based platform that enables the rapid interrogation and large scale screening of human blood antigen presenting cell responses to Toll-like receptor (TLR) ligands and other innate immune modulators. Using this assay, we found that for certain stimuli (e.g., TLR9 and TLR3 ligands), the general protocol for intracellular cytokine cytometry had to be significantly modified to allow response detection. Furthermore, high concentrations of TLR7/8 and TLR4 stimuli caused substantial changes in lineage markers, potentially confounding analysis if one were to use a conventional "lineage-negative" cocktail. The assay we developed is reproducible and has been used to show that a given individual's TLR response pattern is relatively stable over at least several months. This protocol is in strict compliance with published guidelines for polychromatic flow cytometry, provides a common platform for scientists to compare their results directly, and may be applicable to the diagnostic evaluation of Toll-like receptor function and the rapid screening of promising therapeutic innate immune modulators.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.