ArticleJournal of clinical microbiology2008
Evidence of multiple Treponema phylotypes involved in bovine digital dermatitis as shown by 16S rRNA gene analysis and fluorescence in situ hybridization.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Meta-Analysis of Bovine Digital Dermatitis Microbiota Reveals Distinct Microbial Community Structures Associated With Lesions.Frontiers in cellular and infection microbiology · 2021Pooled it
- Effect ofMicrobiology spectrum · 2026Article
- Do Treponemes in Bovine Digital Dermatitis-Associated Claw Horn Lesions Preclude Co-Infection by Pyogenic Bacteria?Microorganisms · 2026Article
- Detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- Review
- Successful implementation of a risk assessment and mitigation program to control bovine digital dermatitis at the herd-level.Scientific reports · 2025Article
- The Effect ofAnimals : an open access journal from MDPI · 2024Article
- Adherence and metal-ion acquisition gene expression increases during infection withInfection and immunity · 2024Article
- Evaluating the impact of application of anaerobic bacterial fermentation enhancer on digital dermatitis.The Journal of veterinary medical science · 2024Article
- Bovine Digital Dermatitis: Treponema spp. on trimming equipment and chutes - effect of washing and disinfection.BMC veterinary research · 2024Article
- Correlation of lesion severity with bacterial changes in Treponeme-Associated Hoof Disease from free-roaming wild elk (Cervus canadensis).Animal microbiome · 2024Article
- Local and Systemic Inflammation in Finnish Dairy Cows with Digital Dermatitis.Animals : an open access journal from MDPI · 2024Article
- Assessment of immunological response to digital dermatitis pathogen derived antigens following infection, recovery, and reinfection.Frontiers in veterinary science · 2024Article
- The microbiota: a key regulator of health, productivity, and reproductive success in mammals.Frontiers in microbiology · 2024Review
- Case report:Frontiers in veterinary science · 2023Article
- Comprehensive Analyses of the Bacterial Population in Non-Healing Claw Lesions of Dairy Cattle.Animals : an open access journal from MDPI · 2022Article
- Detection ofVeterinary research forum : an international quarterly journal · 2022Article
- Treatment of digital dermatitis using salicylic acid in European bison (Frontiers in veterinary science · 2022Article
- First description of interdigital hyperplasia associated with contagious ovine digital dermatitis in two sheep.Frontiers in veterinary science · 2022Article
- "Non-healing" claw horn lesions in dairy cows: Clinical, histopathological and molecular biological characterization of four cases.Frontiers in veterinary science · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The etiopathogenesis of the skin disease digital dermatitis (DD), an important cause of lameness in cattle, remains uncertain. Microscopically, the disease appears to be polymicrobial, with spirochetes as the predominant bacteria. The objective of this study was to identify the main part of the bacteria involved in DD lesions of cattle by using culture-independent molecular methods. Ten different phylotypes of Treponema were identified either by 16S rRNA gene sequencing of bacteria from DD lesions or by fluorescence in situ hybridization (FISH) analysis using phylotype-specific 16S rRNA-directed oligonucleotide probes. Two phylotypes, phylotype 1 (PT1) and PT2, were not closely related to any characterized treponemal species. PT7 was 99.3% identical to Treponema denticola, while PT9 resembled T. vincentii by 96%. The remaining phylotypes, PT3, PT4, PT5, PT6, and PT8, and Treponema brennaborense had previously been isolated from DD lesions. Forty DD biopsy specimens were examined for Treponema by FISH. With one exception, all of the biopsy specimens revealed epidermotropic, intermingled infection with three or more different phylotypes (mean, 4.7). The most prevalent species were PT1 (95%), PT6 (93%), and PT3 (85%). While colonization by PT3 was confined to the surface of the epidermis, both PT1 and PT6 invaded deep into the stratum spinosum and were seen in ulcerated dermal papillae. In two cases, all 10 phylotypes were demonstrated. Furthermore, FISH with a Treponema group-specific probe showed that Treponema accounted for more than 90% of the total bacterial population in the biopsy specimens. These data strongly suggest that a group of apparently symbiotic Treponema species are involved as primary bacterial pathogens in DD.
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