ArticleThe Journal of biological chemistry2008
Quantitative analysis of the high temperature-induced glycolytic flux increase in Saccharomyces cerevisiae reveals dominant metabolic regulation.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Stress-induced remodeling of yeast amino acid pools occurs within conserved physicochemical constraints.Folia microbiologica · 2026Article
- Cost-Effective Solid-State NMR Of Fungal Glucans: A Case Study On Schizosaccharomyces pombe.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Engineering a robust cell-surface display platform in the multi-stress-tolerant yeast Issatchenkia orientalis.Applied microbiology and biotechnology · 2026Article
- Article
- Integrated co-cultivation and subsequent esterification: Harnessing Saccharomyces cerevisiae and Clostridium tyrobutyricum for streamlined ester production.Biotechnology for biofuels and bioproducts · 2025Article
- Calorie Restriction Decreases Competitive Fitness inMicroorganisms · 2024Article
- Simultaneous Nanorheometry and Nanothermometry Using Intracellular Diamond Quantum Sensors.ACS nano · 2023Article
- Shikonin-Loaded Hollow Fe-MOF Nanoparticles for Enhanced Microwave Thermal Therapy.ACS biomaterials science & engineering · 2023Article
- Yeast increases glycolytic flux to support higher growth rates accompanied by decreased metabolite regulation and lower protein phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Parameter inference for enzyme and temperature constrained genome-scale models.Scientific reports · 2023Article
- Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts.Biotechnology for biofuels · 2021Article
- Temperature effect on water dynamics in tetramer phosphofructokinase matrix and the super-arrhenius respiration rate.Scientific reports · 2021Article
- Bayesian genome scale modelling identifies thermal determinants of yeast metabolism.Nature communications · 2021Article
- Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions.Scientific reports · 2020Article
- Yeasts collectively extend the limits of habitable temperatures by secreting glutathione.Nature microbiology · 2020Article
- Selection and subsequent physiological characterization of industrialBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Article
- Temperature induces significant changes in both glycolytic reserve and mitochondrial spare respiratory capacity in colorectal cancer cell lines.Experimental cell research · 2017Article
- Adaptation to different types of stress converge on mitochondrial metabolism.Molecular biology of the cell · 2016Article
- Thermotolerant yeasts selected by adaptive evolution express heat stress response at 30 °C.Scientific reports · 2016Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in systems biology lies in the integration of processes occurring at different levels, such as transcription, translation, and metabolism, to understand the functioning of a living cell in its environment. We studied the high temperature-induced glycolytic flux increase in Saccharomyces cerevisiae and investigated the regulatory mechanisms underlying this increase. We used glucose-limited chemostat cultures to separate regulatory effects of temperature from effects on growth rate. Growth at increased temperature (38 degrees C versus 30 degrees C) resulted in a strongly increased glycolytic flux, accompanied by a switch from respiration to a partially fermentative metabolism. We observed an increased flux through all enzymes, ranging from 5- to 10-fold. We quantified the contributions of direct temperature effects on enzyme activities, the gene expression cascade and shifts in the metabolic network, to the increased flux through each enzyme. To do this we adapted flux regulation analysis. We show that the direct effect of temperature on enzyme kinetics can be included as a separate term. Together with hierarchical regulation and metabolic regulation, this term explains the total flux change between two steady states. Surprisingly, the effect of the cultivation temperature on enzyme catalytic capacity, both directly through the Arrhenius effect and indirectly through adapted gene expression, is only a moderate contribution to the increased glycolytic flux for most enzymes. The changes in flux are therefore largely caused by changes in the interaction of the enzymes with substrates, products, and effectors.
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