ArticleProceedings of the National Academy of Sciences of the United States of America2008
Single-molecule measurements of importin alpha/cargo complex dissociation at the nuclear pore.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 56 citations in OpenAlex.
- Ran modulates allosteric crosstalk between importin β surfaces.Nature communications · 2025Article
- Viral interference of nucleocytoplasmic transport.The Journal of biological chemistry · 2025Review
- 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.APL bioengineering · 2023Article
- Function of the Nuclear Transport Machinery in Maintaining the Distinctive Compositions of the Nucleus and Cytoplasm.International journal of molecular sciences · 2022Review
- Super-resolved 3D tracking of cargo transport through nuclear pore complexes.Nature cell biology · 2022Article
- Speed Microscopy: High-Speed Single Molecule Tracking and Mapping of Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation.Nature protocols · 2021Article
- Cytoplasmic Parvovirus Capsids Recruit Importin Beta for Nuclear Delivery.Journal of virology · 2020Article
- A coarse-grained computational model of the nuclear pore complex predicts Phe-Gly nucleoporin dynamics.The Journal of general physiology · 2017Article
- Article
- Time-resolved biophysical approaches to nucleocytoplasmic transport.Computational and structural biotechnology journal · 2017Review
- Monte Carlo simulations of protein micropatterning in biomembranes: effects of immobile sticky obstacles.Journal of physics D: Applied physics · 2016Article
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
- Super-resolution 3D tomography of interactions and competition in the nuclear pore complex.Nature structural & molecular biology · 2016Article
- SPEED Microscopy and Its Application in Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Review
- MD simulations and FRET reveal an environment-sensitive conformational plasticity of importin-β.Biophysical journal · 2015Article
- Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner.eLife · 2015Article
- Large cargo transport by nuclear pores: implications for the spatial organization of FG-nucleoporins.The EMBO journal · 2013Article
- Defective nuclear import of Tpr in Progeria reflects the Ran sensitivity of large cargo transport.The Journal of cell biology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Macromolecules are transported between the cytoplasm and the nucleoplasm of eukaryotic cells through nuclear pore complexes (NPCs). Large (more than approximately 40 kDa) transport cargoes imported into the nucleus typically form a complex with at least one soluble transport cofactor of the importin (Imp) beta superfamily. Many cargoes require an accessory cofactor, Imp alpha, which binds to Imp beta and to the nuclear localization sequence on the cargo. We previously reported the use of narrow-field epifluorescence microscopy to directly monitor cargoes in transit through NPCs in permeabilized cells. We now report an expanded approach in which single-molecule fluorescence resonance energy transfer (FRET) is used to detect the disassembly of Imp alpha/cargo complexes as they transit through NPCs. We found that CAS, the recycling cofactor for Imp alpha, and RanGTP are essential for this dissociation process. After Imp alpha/cargo complex dissociation, most Imp alpha and cargo molecules entered the nucleoplasm. In contrast, the majority of Imp alpha/cargo complexes that did not dissociate at the NPC in the presence of CAS and RanGTP returned to the cytoplasm. These data are consistent with a model in which Imp alpha/cargo complexes are dissociated on the nucleoplasmic side of the NPC, and this dissociation requires both CAS and RanGTP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.