ArticleProceedings of the National Academy of Sciences of the United States of America2008
Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- EBF1, PAX5, and MYC: regulation on B cell development and association with hematologic neoplasms.Frontiers in immunology · 2024Review
- Hindlimb unloading, a physiological model of microgravity, modifies the murine bone marrow IgM repertoire in a similar manner as aging but less strongly.Immunity & ageing : I & A · 2023Article
- IL-7R signaling activates widespread VCell reports · 2021Article
- The Broad Immunomodulatory Effects of IL-7 and Its Application In Vaccines.Frontiers in immunology · 2021Review
- The histone methyltransferase Setd2 is indispensable for V(D)J recombination.Nature communications · 2019Article
- IL-7 Enables Antibody Responses to Bacterial Polysaccharides by Promoting B Cell Receptor Diversity.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- The SET Domain Is Essential for Metnase Functions in Replication Restart and the 5' End of SS-Overhang Cleavage.PloS one · 2015Article
- Histone methylation and V(D)J recombination.International journal of hematology · 2014Review
- Analysis of chromatin-state plasticity identifies cell-type-specific regulators of H3K27me3 patterns.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Neonatal immunology: responses to pathogenic microorganisms and epigenetics reveal an "immunodiverse" developmental state.Immunologic research · 2013Review
- Deep sequencing of the murine IgH repertoire reveals complex regulation of nonrandom V gene rearrangement frequencies.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Localized epigenetic changes induced by DH recombination restricts recombinase to DJH junctions.Nature immunology · 2012Article
- DNA double-strand breaks relieve USF-mediated repression of Dβ2 germline transcription in developing thymocytes.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Determinants of public T cell responses.Cell research · 2012Review
- Long-distance regulation of fetal V(δ) gene segment TRDV4 by the Tcrd enhancer.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Epigenetic regulation of antigen receptor gene rearrangement.Current opinion in immunology · 2011Review
- Article
- Epigenetic control of recombination in the immune system.Seminars in immunology · 2010Review
- Regulation of antigen receptor gene assembly by genetic-epigenetic crosstalk.Seminars in immunology · 2010Review
- Epigenetic and 3-dimensional regulation of V(D)J rearrangement of immunoglobulin genes.Seminars in immunology · 2010Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The usage of >100 functional murine Ig heavy chain V(H) genes, when rearranged to D(H)J(H) genes, generates a diverse antibody repertoire. The V(H) locus encompasses 2.5 Mb, and rearrangement of V(H) genes in the D(H)-distal half of the locus are controlled very differently from the V(H) genes in the proximal end of the locus. The rearrangement of distal but not proximal V(H) genes is impaired in mice deficient in the cytokine IL-7 or its receptor, in the transcription factor Pax5, or in Ezh2, a histone methyltransferase for Lys-27 of histone H3 (H3K27). The relative role of IL-7, Pax5, and Ezh2 in regulating distal vs. proximal V(H) rearrangement is not clear. Here, we show by ChIP and ChIP-on-chip that the active histone modification H3K36me2 is most highly associated with distal V(H) segments and the repressive histone modification H3K27me3 is exclusively present on proximal V(H) segments. We observed an absence of H3K27me3 in fetal pro-B cells, which predominantly rearrange proximal V(H) genes. Absence of IL-7 signaling reduces H3K36me2, and overexpression of IL-7 increases H3K36me2. In contrast, the major effect of the absence of Pax5 is the reduction in H3K27me3. Our data indicate that the cytokine IL-7 and the transcription factor Pax5 influence the rearrangement of the two regions of the V(H) locus by differentially modulating two reciprocal histone modifications during B lymphocyte development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.