ArticleCell motility and the cytoskeleton2008
Sarcomere alignment is regulated by myocyte shape.
Article in Cell motility and the cytoskeleton, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers.
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Who cites it
130 citing papers in PubMed, 229 citations in OpenAlex.
- Restoring anisotropy after myocardial injury: strategies to align transplanted human induced pluripotent stem-cell-derived cardiomyocytes.Experimental & molecular medicine · 2026Review
- Review
- Cardiac mechanotransduction from development to disease.APL bioengineering · 2026Review
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- Cell shape and maturation impacts α-actinin-2 tension in iPSC-derived cardiomyocytes.APL bioengineering · 2026Article
- Nanopatterned Cell Sheet Assembly of Biomimetic Cardiac Laminae for Modeling Structure-Function Relationships.Biomaterials research · 2026Article
- Collagen XV preserves heart function and protects from pathological remodelling after myocardial infarction.The FEBS journal · 2026Article
- Extracellular matrix alterations in chronic ischemic cardiomyopathy revealed by quantitative proteomics.JCI insight · 2025Article
- Myocardial disarray drives metabolic inefficiency in human cardiomyocytes.Communications biology · 2025Article
- Quantifying HiPSC-CM structural organization at scale with deep learning-enhanced SarcGraph.PLoS computational biology · 2025Article
- Numerical models for organ-on-a-chip: A systematic review and analyses.Biomicrofluidics · 2025Review
- Virally delivered CMYA5 enhances the assembly of cardiac dyads.Nature biomedical engineering · 2025Article
- Dysregulation of N-terminal acetylation causes cardiac arrhythmia and cardiomyopathy.Nature communications · 2025Article
- Cell Architecture and Dynamics of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) on Hydrogels with Spatially Patterned Laminin and N-Cadherin.ACS applied materials & interfaces · 2025Article
- Advancing Cardiac Organoid Engineering Through Application of Biophysical Forces.IEEE reviews in biomedical engineering · 2024Article
- Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cells.Stem cell reports · 2024Article
- Diffusion model predicts the geometry of actin cytoskeleton from cell morphology.PLoS computational biology · 2024Article
- Efficient and reproducible generation of human iPSC-derived cardiomyocytes and cardiac organoids in stirred suspension systems.Nature communications · 2024Article
- Selective Induction of Molecular Assembly to Tissue-Level Anisotropy on Peptide-Based Optoelectronic Cardiac Biointerfaces.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Efficient and reproducible generation of human iPSC-derived cardiomyocytes using a stirred bioreactor.bioRxiv : the preprint server for biology · 2024Article
70 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cardiac organogenesis and pathogenesis are both characterized by changes in myocyte shape, cytoskeletal architecture, and the extracellular matrix (ECM). However, the mechanisms by which the ECM influences myocyte shape and myofibrillar patterning are unknown. We hypothesized that geometric cues in the ECM align sarcomeres by directing the actin network orientation. To test our hypothesis, we cultured neonatal rat ventricular myocytes on islands of micro-patterned ECM to measure how they remodeled their cytoskeleton in response to extracellular cues. Myocytes spread and assumed the shape of circular and rectangular islands and reorganized their cytoskeletons and myofibrillar arrays with respect to the ECM boundary conditions. Circular myocytes did not assemble predictable actin networks nor organized sarcomere arrays. In contrast, myocytes cultured on rectangular ECM patterns with aspect ratios ranging from 1:1 to 7:1 aligned their sarcomeres in predictable and repeatable patterns based on highly localized focal adhesion complexes. Examination of averaged alpha-actinin images revealed invariant sarcomeric registration irrespective of myocyte aspect ratio. Since the sarcomere sub-units possess a fixed length, this observation indicates that cytoskeleton configuration is length-limited by the extracellular boundary conditions. These results indicate that modification of the extracellular microenvironment induces dynamic reconfiguring of the myocyte shape and intracellular architecture. Furthermore, geometric boundaries such as corners induce localized myofibrillar anisotropy that becomes global as the myocyte aspect ratio increases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.