ArticleEnvironmental health perspectives2008
Transcriptome analyses in normal prostate epithelial cells exposed to low-dose cadmium: oncogenic and immunomodulations involving the action of tumor necrosis factor.
Article in Environmental health perspectives, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 57 citations in OpenAlex.
- Prostate cancer microenvironment: multidimensional regulation of immune cells, vascular system, stromal cells, and microbiota.Molecular cancer · 2024Review
- Cadmium-induced Carcinogenesis in Respiratory Organs and the Prostate: Insights from Three Perspectives on Toxicogenomic Approach.Journal of cancer prevention · 2023Review
- Phenotype of Atopic Dermatitis With Food Allergy Predicts Development of Childhood Asthma via Gut Wnt Signaling.Allergy, asthma & immunology research · 2022Article
- Effects of arsenic and heavy metals on metabolic pathways in cells of human origin: Similarities and differences.Toxicology reports · 2021Article
- Infection of Monocytes From Tuberculosis Patients With Two Virulent Clinical Isolates ofFrontiers in cellular and infection microbiology · 2020Article
- Intracerebral Hemorrhage Induces Inflammatory Gene Expression in Peripheral Blood: Global Transcriptional Profiling in Intracerebral Hemorrhage Patients.DNA and cell biology · 2019Article
- Intracerebral hemorrhage induces monocyte-related gene expression within six hours: Global transcriptional profiling in swine ICH.Metabolic brain disease · 2019Article
- Loss of DIP2C in RKO cells stimulates changes in DNA methylation and epithelial-mesenchymal transition.BMC cancer · 2017Article
- Cadmium promotes the proliferation of triple-negative breast cancer cells through EGFR-mediated cell cycle regulation.Toxicology and applied pharmacology · 2015Article
- Exposure of Human Prostaspheres to Bisphenol A Epigenetically Regulates SNORD Family Noncoding RNAs via Histone Modification.Endocrinology · 2015Article
- Replication factor C3 is a CREB target gene that regulates cell cycle progression through the modulation of chromatin loading of PCNA.Leukemia · 2015Article
- Cadmium down-regulates expression of XIAP at the post-transcriptional level in prostate cancer cells through an NF-kappaB-independent, proteasome-mediated mechanism.Molecular cancer · 2010Article
- Cadmium induces lung inflammation independent of lung cell proliferation: a molecular approach.Journal of inflammation (London, England) · 2009Article
- Deletion hotspots in AMACR promoter CpG island are cis-regulatory elements controlling the gene expression in the colon.PLoS genetics · 2009Article
- Cadmium increases ferroportin-1 gene expression in J774 macrophage cells via the production of reactive oxygen species.Nutrition research and practice · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCadmium is implicated in prostate carcinogenesis, but its oncogenic action remains unclear.
objectivesIn this study we aimed to decipher changes in cell growth and the transcriptome in an immortalized human normal prostate epithelial cell line (NPrEC) following exposure to low-dose Cd.
methodsSynchronized NPrEC cells were exposed to different doses of Cd and assayed for cell viability and cell-cycle progression. We investigated changes in transcriptome by global profiling and used Ingenuity Pathways Analysis software to develop propositions about functional connections among differentially expressed genes. A neutralizing antibody was used to negate the effect of Cd-induced up-regulation of tumor necrosis factor (TNF) in NPrEC cells.
resultsExposure of NPrEC to 2.5 microM Cd enhanced cell viability and accelerated cell-cycle progression. Global expression profiling identified 48 genes that exhibited >or= 1.5-fold changes in expression after 4, 8, 16, and 32 hr of Cd treatment. Pathway analyses inferred a functional connection among 35 of these genes in one major network, with TNF as the most prominent node. Fourteen of the 35 genes are related to TNF, and 11 exhibited an average of >2-fold changes in gene expression. Real-time reverse transcriptase-polymerase chain reaction confirmed the up-regulation of 7 of the 11 genes (ADAM8, EDN1, IL8, IL24, IL13RA2, COX2/PTGS2, and SERPINB2) and uncovered a 28-fold transient increase in TNF expression in Cd-treated NPrEC cells. A TNF-neutralizing antibody effectively blocked Cd-induced elevations in the expression of these genes.
conclusionsNoncytotoxic, low-dose Cd has growth-promoting effects on NPrEC cells and induces transient overexpression of TNF, leading to up-regulation of genes with oncogenic and immunomodulation functions.
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