ArticlePloS one2008
A microfluidic chamber for analysis of neuron-to-cell spread and axonal transport of an alpha-herpesvirus.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed.
- Ex vivo study of neuroinvasive and neurotropic viruses: what is current and what is next.FEMS microbiology reviews · 2025Review
- Article
- Engineering organ-on-a-chip systems to model viral infections.Biofabrication · 2023Review
- Generation of stable advective-diffusive chemokine gradients in a three-dimensional hydrogel.AIP advances · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Herpes Simplex Virus 2 Counteracts Neurite Outgrowth Repulsion during Infection in a Nerve Growth Factor-Dependent Manner.Journal of virology · 2020Article
- Contemporary Circulating Enterovirus D68 Strains Infect and Undergo Retrograde Axonal Transport in Spinal Motor Neurons Independent of Sialic Acid.Journal of virology · 2019Article
- Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.Neuroscience bulletin · 2019Article
- The Carboxyl Terminus of Tegument Protein pUL21 Contributes to Pseudorabies Virus Neuroinvasion.Journal of virology · 2019Article
- Latent versus productive infection: the alpha herpesvirus switch.Future virology · 2018Review
- Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine.Stem cell reviews and reports · 2017Review
- Glia-neuron interactions in neurological diseases: Testing non-cell autonomy in a dish.Brain research · 2017Review
- A Therapeutic Antiviral Antibody Inhibits the Anterograde Directed Neuron-to-Cell Spread of Herpes Simplex Virus and Protects against Ocular Disease.Frontiers in microbiology · 2017Article
- Structure, Function, and Propagation of Information across Living Two, Four, and Eight Node Degree Topologies.Frontiers in bioengineering and biotechnology · 2016Article
- Feed-Forward Propagation of Temporal and Rate Information between Cortical Populations during Coherent Activation in Engineered In Vitro Networks.Frontiers in neural circuits · 2016Article
- Histone acetylation inhibitors promote axon growth in adult dorsal root ganglia neurons.Journal of neuroscience research · 2015Article
- Us9-Independent Axonal Sorting and Transport of the Pseudorabies Virus Glycoprotein gM.Journal of virology · 2015Article
- Article
- An in vitro method to manipulate the direction and functional strength between neural populations.Frontiers in neural circuits · 2015Review
- Direct transfer of viral and cellular proteins from varicella-zoster virus-infected non-neuronal cells to human axons.PloS one · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Alpha-herpesviruses, including herpes simplex virus and pseudorabies virus (PRV), infect the peripheral nervous system (PNS) of their hosts. Here, we describe an in vitro method for studying neuron-to-cell spread of infection as well as viral transport in axons. The method centers on a novel microfluidic chamber system that directs growth of axons into a fluidically isolated environment. The system uses substantially smaller amounts of virus inoculum and media than previous chamber systems and yet offers the flexibility of applying multiple virology and cell biology assays including live-cell optical imaging. Using PRV infection of cultured PNS neurons, we demonstrate that the microfluidic chamber recapitulates all known facets of neuron-to-cell spread demonstrated in animals and other compartmented cell systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.