ArticleGenomics, proteomics & bioinformatics2008
Transcriptional analysis of normal human fibroblast responses to microgravity stress.
Article in Genomics, proteomics & bioinformatics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Women in space: A review of known physiological adaptations and health perspectives.Experimental physiology · 2026Review
- PI3K/AKT/mTOR pathway and c-Myc inhibit hepatocyte suspension under simulated microgravity.NPJ microgravity · 2025Article
- Oxidative Stress on the Ground and in the Microgravity Environment: Pathophysiological Effects and Treatment.Antioxidants (Basel, Switzerland) · 2025Review
- Hydrogel mechanical properties in altered gravity.NPJ microgravity · 2024Article
- Review
- Single-cell multi-ome and immune profiles of the Inspiration4 crew reveal conserved, cell-type, and sex-specific responses to spaceflight.Nature communications · 2024Article
- Hypergravity Attenuates Reactivity in Primary Murine Astrocytes.Biomedicines · 2022Article
- Adaptation and Changes in Actin Dynamics and Cell Motility as Early Responses of Cultured Mammalian Cells to Altered Gravitational Vector.International journal of molecular sciences · 2022Article
- Role of fibroblasts in wound healing and tissue remodeling on Earth and in space.Frontiers in bioengineering and biotechnology · 2022Review
- Fibroblast Differentiation and Matrix Remodeling Impaired under Simulated Microgravity in 3D Cell Culture Model.International journal of molecular sciences · 2021Article
- Genomic Changes Driven by Radiation-Induced DNA Damage and Microgravity in Human Cells.International journal of molecular sciences · 2021Review
- Role of Apoptosis in Wound Healing and Apoptosis Alterations in Microgravity.Frontiers in bioengineering and biotechnology · 2021Review
- Spaceflight-Induced Bone Tissue Changes that Affect Bone Quality and Increase Fracture Risk.Current osteoporosis reports · 2020Review
- Effects of Spaceflight and Simulated Microgravity on YAP1 Expression in Cardiovascular Progenitors: Implications for Cell-Based Repair.International journal of molecular sciences · 2019Article
- The Trends in Global Gene Expression in Mouse Embryonic Stem Cells During Spaceflight.Frontiers in genetics · 2019Article
- Towards human exploration of space: the THESEUS review series on muscle and bone research priorities.NPJ microgravity · 2017Article
- The eIF4E2-Directed Hypoxic Cap-Dependent Translation Machinery Reveals Novel Therapeutic Potential for Cancer Treatment.Oxidative medicine and cellular longevity · 2017Review
- Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.Scientific reports · 2016Article
- A systems biology pipeline identifies new immune and disease related molecular signatures and networks in human cells during microgravity exposure.Scientific reports · 2016Article
- Mechanotransduction as an Adaptation to Gravity.Frontiers in pediatrics · 2016Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the molecular mechanism(s) of how spaceflight affects cellular signaling pathways, quiescent normal human WI-38 fibroblasts were flown on the STS-93 space shuttle mission. Subsequently, RNA samples from the space-flown and ground-control cells were used to construct two cDNA libraries, which were then processed for suppression subtractive hybridization (SSH) to identify spaceflight-specific gene expression. The SSH data show that key genes related to oxidative stress, DNA repair, and fatty acid oxidation are activated by spaceflight, suggesting the induction of cellular oxidative stress. This is further substantiated by the up-regulation of neuregulin 1 and the calcium-binding protein calmodulin 2. Another obvious stress sign is that spaceflight evokes the Ras/mitogen-activated protein kinase and phosphatidylinositol-3 kinase signaling pathways, along with up-regulating several G1-phase cell cycle traverse genes. Other genes showing up-regulation of expression are involved in protein synthesis and pro-apoptosis, as well as pro-survival. Interactome analysis of functionally related genes shows that c-Myc is the "hub" for those genes showing significant changes. Hence, our results suggest that microgravity travel may impact changes in gene expression mostly associated with cellular stress signaling, directing cells to either apoptotic death or premature senescence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.