ArticleGastroenterology2008
Constitutive CXCL12 expression induces anoikis in colorectal carcinoma cells.
Article in Gastroenterology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Unraveling the anoikis-cancer nexus: a bibliometric analysis of research trends and mechanisms.Future science OA · 2025Article
- Pokemon inhibits Bim transcription to promote the proliferation, anti-anoikis, invasion, histological grade, and dukes stage of colorectal neoplasms.Journal of cancer research and clinical oncology · 2024Article
- Review
- The liver metastatic niche: modelling the extracellular matrix in metastasis.Disease models & mechanisms · 2021Review
- Molecular mediators of peritoneal metastasis in pancreatic cancer.Cancer metastasis reviews · 2020Review
- Neutrophil-Derived Reactive Oxygen Orchestrates Epithelial Cell Signaling Events during Intestinal Repair.The American journal of pathology · 2019Article
- The role of vitamin D in hepatic metastases from colorectal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2018Review
- Computational Model for Predicting the Relationship Between Micro-RNAs and Their Target Messenger RNAs in Breast and Colon Cancers.Cancer informatics · 2018Article
- Histone hypoacetylation contributes to CXCL12 downregulation in colon cancer: impact on tumor growth and cell migration.Oncotarget · 2017Article
- CXCL12 methylation-mediated epigenetic regulation of gene expression in papillary thyroid carcinoma.Scientific reports · 2017Article
- Adiponectin deficiency contributes to the development and progression of benign prostatic hyperplasia in obesity.Scientific reports · 2017Article
- Exploiting agonist biased signaling of chemokines to target cancer.Molecular carcinogenesis · 2017Article
- Cyclic AMP regulates the migration and invasion potential of human pancreatic cancer cells.Molecular carcinogenesis · 2015Article
- Prognostic value of stromal cell-derived factor 1 expression in patients with gastric cancer after surgical resection.Cancer science · 2014Article
- CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Chemokines and chemokine receptors: update on utility and challenges for the clinician.Surgery · 2014Review
- AEG-1 promotes anoikis resistance and orientation chemotaxis in hepatocellular carcinoma cells.PloS one · 2014Article
- CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis.PloS one · 2014Article
- Down-regulation of CXCL12 by DNA hypermethylation and its involvement in gastric cancer metastatic progression.Digestive diseases and sciences · 2012Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND &
aimsCXCL12 and CXCR4 signaling plays critical roles in development, homeostasis, and tumor metastasis. Previously, we have shown that epigenetic silencing of CXCL12 in colorectal and mammary carcinomas promotes metastasis. Anoikis is an essential process of colonic epithelial turnover and limits the metastatic progression of carcinoma. We sought to determine the role for anoikis in limiting tumor metastasis following reexpression of CXCL12 in human colorectal carcinoma cells.
methodsTumor formation and metastasis of colonic carcinoma cells was monitored using in vivo bioluminescence imaging. Anoikis was defined by using caspase-3/7, focal adhesion kinase (FAK) and p130Cas cleavage, DNA fragmentation, and cell survival assays. Phosphorylation of extracellular-regulated kinase-1/2 (ERK1/2) was monitored by immunoblot and immunohistochemistry, and activity was inhibited by using U0126.
resultsConstitutive expression of CXCL12 in human colorectal carcinoma cells reduced orthotopic tumor formation and inhibited metastasis in severe combined immunodeficient mice. Further, CXCL12 expression induced apoptosis specifically in nonadherent colorectal carcinoma cells. Apoptotic cell death was preceded by hypophosphorylation and cleavage of FAK and p130Cas, leading to increased cellular detachment in culture, and depended on alterations in the extracellular matrix. Similar to in vivo colonic epithelium, CXCL12-induced anoikis of carcinoma cells depended on basal ERK1/2 activation.
conclusionsThese data significantly expand the current paradigm of chemokine signaling in carcinogenesis by showing that endogenous CXCL12, in marked contrast to exogenous ligand, inhibits tumor metastasis through increased anoikis. Altered ERK1/2 signaling provides a mechanism for the dichotomy between the physiologic and pathophysiologic roles of CXCL12-CXCR4 signaling in the intestinal epithelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.