ArticleNeurogastroenterology and motility2008
Regulation of SRF/CArG-dependent gene transcription during chronic partial obstruction of murine small intestine.
Article in Neurogastroenterology and motility, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Visceral myopathy: clinical syndromes, genetics, pathophysiology, and fall of the cytoskeleton.American journal of physiology. Gastrointestinal and liver physiology · 2021Review
- Gastroparesis is associated with decreased FOXF1 and FOXF2 in humans, and loss of FOXF1 and FOXF2 results in gastroparesis in mice.Neurogastroenterology and motility · 2019Article
- A Mouse Model of Intestinal Partial Obstruction.Journal of visualized experiments : JoVE · 2018Article
- Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures.PloS one · 2017Article
- Multi-phenotypic Role of Serum Response Factor in the Gastrointestinal System.Journal of neurogastroenterology and motility · 2016Review
- Molecular and Cellular Characteristics of the Colonic Pseudo-obstruction in Patients With Intractable Constipation.Journal of neurogastroenterology and motility · 2015Article
- Serum Response Factor Is Essential for Prenatal Gastrointestinal Smooth Muscle Development and Maintenance of Differentiated Phenotype.Journal of neurogastroenterology and motility · 2015Article
- Early Adaptation of Small Intestine After Massive Small Bowel Resection in Rats.Iranian journal of pediatrics · 2015Article
- Smooth Muscle Cell Genome Browser: Enabling the Identification of Novel Serum Response Factor Target Genes.PloS one · 2015Article
- Myocardin in biology and disease.Journal of biomedical research · 2015Review
- The transcription factor Foxf1 binds to serum response factor and myocardin to regulate gene transcription in visceral smooth muscle cells.The Journal of biological chemistry · 2013Article
- Disruption of interstitial cells of Cajal networks after massive small bowel resection.World journal of gastroenterology · 2013Article
- Epigenetics and chromatin dynamics: a review and a paradigm for functional disorders.Neurogastroenterology and motility · 2012Review
- Article
- Stress and strain analysis of contractions during ramp distension in partially obstructed guinea pig jejunal segments.Journal of biomechanics · 2011Article
- Hedgehog signaling controls homeostasis of adult intestinal smooth muscle.Developmental biology · 2011Article
- Regulation of gastrointestinal motility by Ca2+/calmodulin-stimulated protein kinase II.Archives of biochemistry and biophysics · 2011Review
- Phasic and tonic smooth muscle function of the partially obstructed guinea pig intestine.Journal of biomedicine & biotechnology · 2011Article
- Biomechanical remodelling of obstructed guinea pig jejunum.Journal of biomechanics · 2010Article
- Cellular pathogenesis of diabetic gastroenteropathy.Minerva gastroenterologica e dietologica · 2009Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intestinal obstructions lead to a variety of motility disorders. Small intestine smooth muscles undergo dramatic phenotypic changes in response to obstruction, but the underlying molecular mechanisms are unknown. Using RT-PCR, ChIP, Re-ChIP, and Western blots, we examined the effect of small bowel mechanical obstruction on smooth muscle gene expression. Obstruction caused a transient hyperplasia, followed by a prolonged hypertrophic response of small intestine smooth muscle cells. Smooth muscle myosin heavy chain (MHC), alpha-actin, and gamma-actin expression decreased initially, and then increased as hypertrophy developed. Myocardin expression decreased initially and then increased, while kruppel-like factors (KLF)4 and KLF5 expression increased initially, and then decreased. Serum response factor (SRF) expression decreased initially, and then recovered to sham-operated levels as hypertrophy developed. SRF binding to smooth muscle MHC and alpha-actin promoters decreased initially, but then increased above sham-operated levels as hypertrophy developed. Elk-1 binding to smooth muscle myosin heavy chain and alpha-actin promoters increased initially, and then decreased to sham-operated levels as hypertrophy developed. c-fos expression increased initially, which was associated with increased SRF/Elk-1 binding to the c-fos promoter. The Elk-1 phosphorylation inhibitor U-0126 inhibited the increase in c-fos expression. These findings indicate a dynamic response of small intestine smooth muscles to bowel obstruction involving switching between differentiated, proliferative, and hypertrophic phenotypes. These results suggest that changes in the expression and interactions between SRF, myocardin, Elk-1, and c-fos play key roles in the phenotypic switching of small intestine smooth muscles in response to mechanical obstruction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.