ArticleJournal of bacteriology2008
Regulation of autolysis-dependent extracellular DNA release by Enterococcus faecalis extracellular proteases influences biofilm development.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
147 citing papers in PubMed, 303 citations in OpenAlex.
- Trial
- The protease code of the bacterial gut microbiota: ecological regulation, barrier disruption, and rewiring host defense and signaling.Gut microbes · 2026Review
- Molecular Regulation of Virulence and Dental Biofilm Formation in Streptococcus mutans: Unravelling the Crosstalk Between the VicRK and LiaSR Two-Component Systems.Molecular oral microbiology · 2026Review
- De novo or Salvage? Nucleotide Availability as a Driver of Bacterial Adaptation and Virulence.MicrobiologyOpen · 2026Review
- Loss of Fsr quorum sensing promotes biofilm formation and worsens outcomes in enterococcal infective endocarditis.Nature communications · 2026Article
- Sanitization of food-grade materials: Combined effect of an enzymatic product and two chemical sanitizers onAIMS microbiology · 2026Article
- Effect of Chemical Sanitizers Alone or Combined with An Enzymatic Product Against Pseudomonas fluorescens Biofilms on Food-Grade Materials.Current microbiology · 2025Article
- The natural symbiotic bacterium Enterococcus faecalis LX10 drives Bombyx mori refractoriness to Nosema bombycis infection via the secretion of enterococcin.BMC microbiology · 2025Article
- Pleural Infection: Diagnosis, Management, and Future Directions.Journal of clinical medicine · 2025Review
- Capturing the micro-communities: Insights into biogenesis and architecture of bacterial biofilms.BBA advances · 2025Article
- Polidocanol inhibits Enterococcus faecalis virulence factors by targeting fsr quorum sensing system.BMC microbiology · 2024Article
- Biofilm matrix: a multifaceted layer of biomolecules and a defensive barrier against antimicrobials.Archives of microbiology · 2024Review
- Triple threat: how diabetes results in worsened bacterial infections.Infection and immunity · 2024Review
- Non-Clinical microbiology reviews · 2024Review
- Enterococcal quorum-controlled protease alters phage infection.bioRxiv : the preprint server for biology · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Comparative Analysis of Antibiotic Resistance and Biofilm Characteristics of Two MajorVeterinary sciences · 2024Article
- Bacterial biofilm growth and perturbation by serine protease from Bacillus sp.Archives of microbiology · 2024Article
- Strategies and mechanisms targetingFrontiers in cellular and infection microbiology · 2024Review
- Enterococcal quorum-controlled protease alters phage infection.FEMS microbes · 2024Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterococci are major contributors of hospital-acquired infections and have emerged as important reservoirs for the dissemination of antibiotic resistance traits. The ability to form biofilms on medical devices is an important aspect of pathogenesis in the hospital environment. The Enterococcus faecalis Fsr quorum system has been shown to regulate biofilm formation through the production of gelatinase, but the mechanism has been hitherto unknown. Here we show that both gelatinase (GelE) and serine protease (SprE) contribute to biofilm formation by E. faecalis and provide clues to how the activity of these proteases governs this developmental process. Confocal imaging of biofilms suggested that GelE(-) mutants were significantly reduced in biofilm biomass compared to the parental strain, whereas the absence of SprE appeared to accelerate the progression of biofilm development. The phenotype observed in a SprE(-) mutant was linked to an observed increase in autolytic rate compared to the parental strain. Culture supernatant analysis and confocal microscopy confirmed the inability of mutants deficient in GelE to release extracellular DNA (eDNA) in planktonic and biofilm cultures, whereas cells deficient in SprE produced significantly more eDNA as a component of the biofilm matrix. DNase I treatment of E. faecalis biofilms reduced the accumulation of biofilm, implying a critical role for eDNA in biofilm development. In conclusion, our data suggest that the interplay of two secreted and coregulated proteases--GelE and SprE--is responsible for regulating autolysis and the release of high-molecular-weight eDNA, a critical component for the development of E. faecalis biofilms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.