ArticleGenome biology2008
Identification of transcripts with enriched expression in the developing and adult pancreas.
Article in Genome biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- Colorectal Cancer-Associated Genes Are Associated with Tooth Agenesis and May Have a Role in Tooth Development.Scientific reports · 2018Article
- Postimplantation Mga expression and embryonic lethality of two gene-trap alleles.Gene expression patterns : GEP · 2018Article
- Glucose tolerance female-specific QTL mapped in collaborative cross mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Article
- Single cell transcriptomic profiling of mouse pancreatic progenitors.Physiological genomics · 2017Article
- Myt3 suppression sensitizes islet cells to high glucose-induced cell death via Bim induction.Cell death & disease · 2016Article
- Article
- PDX1 binds and represses hepatic genes to ensure robust pancreatic commitment in differentiating human embryonic stem cells.Stem cell reports · 2015Article
- Article
- An integrated cell purification and genomics strategy reveals multiple regulators of pancreas development.PLoS genetics · 2014Article
- Profiling of embryonic stem cell differentiation.The review of diabetic studies : RDS · 2014Review
- Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- The pancreatic β cell and type 1 diabetes: innocent bystander or active participant?Trends in endocrinology and metabolism: TEM · 2013Article
- Identification and analysis of murine pancreatic islet enhancers.Diabetologia · 2013Article
- Microarray analysis of novel candidate genes responsible for glucose-stimulated insulin secretion in mouse pancreatic β cell line MIN6.PloS one · 2013Article
- Article
- Purines as potential morphogens during embryonic development.Purinergic signalling · 2012Review
- Mouse strain specific gene expression differences for illumina microarray expression profiling in embryos.BMC research notes · 2012Article
- Article
- Targeting SOX17 in human embryonic stem cells creates unique strategies for isolating and analyzing developing endoderm.Cell stem cell · 2011Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite recent advances, the transcriptional hierarchy driving pancreas organogenesis remains largely unknown, in part due to the paucity of comprehensive analyses. To address this deficit we generated ten SAGE libraries from the developing murine pancreas spanning Theiler stages 17-26, making use of available Pdx1 enhanced green fluorescent protein (EGFP) and Neurog3 EGFP reporter strains, as well as tissue from adult islets and ducts.
resultsWe used a specificity metric to identify 2,536 tags with pancreas-enriched expression compared to 195 other mouse SAGE libraries. We subsequently grouped co-expressed transcripts with differential expression during pancreas development using K-means clustering. We validated the clusters first using quantitative real time PCR and then by analyzing the Theiler stage 22 pancreas in situ hybridization staining patterns of over 600 of the identified genes using the GenePaint database. These were then categorized into one of the five expression domains within the developing pancreas. Based on these results we identified a cascade of transcriptional regulators expressed in the endocrine pancreas lineage and, from this, we developed a predictive regulatory network describing beta-cell development.
conclusionTaken together, this work provides evidence that the SAGE libraries generated here are a valuable resource for continuing to elucidate the molecular mechanisms regulating pancreas development. Furthermore, our studies provide a comprehensive analysis of pancreas development, and insights into the regulatory networks driving this process are revealed.
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