ArticleBMC plant biology2008
A physical map of the heterozygous grapevine 'Cabernet Sauvignon' allows mapping candidate genes for disease resistance.
Article in BMC plant biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 69 citations in OpenAlex.
- Multivirulent Plasmopara viticola strains from Cilaos on Réunion Island break down Rpv1, Rpv3.1 and Rpv10 mediated resistance of grapevine.BMC plant biology · 2026Article
- TheFrontiers in plant science · 2019Article
- Quantitative trait loci affecting pathogen resistance and ripening of grapevines.Molecular genetics and genomics : MGG · 2016Article
- A genetic map of cassava (Manihot esculenta Crantz) with integrated physical mapping of immunity-related genes.BMC genomics · 2015Article
- Current understanding of grapevine defense mechanisms against the biotrophic fungus (Erysiphe necator), the causal agent of powdery mildew disease.Horticulture research · 2015Review
- Functions of EDS1-like and PAD4 genes in grapevine defenses against powdery mildew.Plant molecular biology · 2014Article
- Grapevine powdery mildew resistance and susceptibility loci identified on a high-resolution SNP map.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Mining of haplotype-based expressed sequence tag single nucleotide polymorphisms in citrus.BMC genomics · 2013Article
- Validation assay of p3_VvAGL11 marker in a wide range of genetic background for early selection of stenospermocarpy in Vitis vinifera L.Molecular biotechnology · 2013Article
- Historical introgression of the downy mildew resistance gene Rpv12 from the Asian species Vitis amurensis into grapevine varieties.PloS one · 2013Article
- A reference genetic map of Muscadinia rotundifolia and identification of Ren5, a new major locus for resistance to grapevine powdery mildew.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- A framework genetic map of Muscadinia rotundifolia.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Genomic organisation of the Mal d 1 gene cluster on linkage group 16 in apple.Molecular breeding : new strategies in plant improvement · 2012Article
- Selective sweep at the Rpv3 locus during grapevine breeding for downy mildew resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference.Plant cell reports · 2012Article
- Development of marker sets useful in the early selection of Ren4 powdery mildew resistance and seedlessness for table and raisin grape breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- A hybrid BAC physical map of potato: a framework for sequencing a heterozygous genome.BMC genomics · 2011Article
- Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Using a limited mapping strategy to identify major QTLs for resistance to grapevine powdery mildew (Erysiphe necator) and their use in marker-assisted breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Construction of papaya male and female BAC libraries and application in physical mapping of the sex chromosomes.Journal of biomedicine & biotechnology · 2011Article
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhole-genome physical maps facilitate genome sequencing, sequence assembly, mapping of candidate genes, and the design of targeted genetic markers. An automated protocol was used to construct a Vitis vinifera 'Cabernet Sauvignon' physical map. The quality of the result was addressed with regard to the effect of high heterozygosity on the accuracy of contig assembly. Its usefulness for the genome-wide mapping of genes for disease resistance, which is an important trait for grapevine, was then assessed.
resultsThe physical map included 29,727 BAC clones assembled into 1,770 contigs, spanning 715,684 kbp, and corresponding to 1.5-fold the genome size. Map inflation was due to high heterozygosity, which caused either the separation of allelic BACs in two different contigs, or local mis-assembly in contigs containing BACs from the two haplotypes. Genetic markers anchored 395 contigs or 255,476 kbp to chromosomes. The fully automated assembly and anchorage procedures were validated by BAC-by-BAC blast of the end sequences against the grape genome sequence, unveiling 7.3% of chimerical contigs. The distribution across the physical map of candidate genes for non-host and host resistance, and for defence signalling pathways was then studied. NBS-LRR and RLK genes for host resistance were found in 424 contigs, 133 of them (32%) were assigned to chromosomes, on which they are mostly organised in clusters. Non-host and defence signalling genes were found in 99 contigs dispersed without a discernable pattern across the genome.
conclusionDespite some limitations that interfere with the correct assembly of heterozygous clones into contigs, the 'Cabernet Sauvignon' physical map is a useful and reliable intermediary step between a genetic map and the genome sequence. This tool was successfully exploited for a quick mapping of complex families of genes, and it strengthened previous clues of co-localisation of major NBS-LRR clusters and disease resistance loci in grapevine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.