ArticleBMC microbiology2008
Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Extensive re-modelling of the cell wall during the development ofbioRxiv : the preprint server for biology · 2023Article
- Egress of Listeria monocytogenes from Mesenteric Lymph Nodes Depends on Intracellular Replication and Cell-to-Cell Spread.Infection and immunity · 2023Article
- Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.The ISME journal · 2022Article
- Msh Pilus Mutations Increase the Ability of a Free-Living Bacterium to Colonize a Piscine Host.Genes · 2021Article
- The Small Protein YmoA Controls the Csr System and Adjusts Expression of Virulence-Relevant Traits ofFrontiers in microbiology · 2021Article
- Chaperonin Abundance Enhances Bacterial Fitness.Frontiers in molecular biosciences · 2021Article
- Monocytes Are the Predominant Cell Type Associated withJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Uncovering Listeria monocytogenes hypervirulence by harnessing its biodiversity.Nature genetics · 2016Article
- Intracellular Listeria monocytogenes comprises a minimal but vital fraction of the intestinal burden following foodborne infection.Infection and immunity · 2015Article
- Tropical strains of Ralstonia solanacearum Outcompete race 3 biovar 2 strains at lowland tropical temperatures.Applied and environmental microbiology · 2015Article
- The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.The Journal of biological chemistry · 2014Article
- Essential role of invasin for colonization and persistence of Yersinia enterocolitica in its natural reservoir host, the pig.Infection and immunity · 2014Article
- The RNA chaperone Hfq is required for virulence of Bordetella pertussis.Infection and immunity · 2013Article
- Hyperinduction of host beta interferon by a Listeria monocytogenes strain naturally overexpressing the multidrug efflux pump MdrT.Infection and immunity · 2012Article
- In vivo-induced InvA-like autotransporters Ifp and InvC of Yersinia pseudotuberculosis promote interactions with intestinal epithelial cells and contribute to virulence.Infection and immunity · 2012Article
- Article
- InlA promotes dissemination of Listeria monocytogenes to the mesenteric lymph nodes during food borne infection of mice.PLoS pathogens · 2012Article
- Crp induces switching of the CsrB and CsrC RNAs in Yersinia pseudotuberculosis and links nutritional status to virulence.Frontiers in cellular and infection microbiology · 2012Article
- Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe foodborne, gram-positive pathogen, Listeria monocytogenes, is capable of causing lethal infections in compromised individuals. In the post genomic era of L. monocytogenes research, techniques are required to identify and validate genes involved in the pathogenicity and environmental biology of the organism. The aim here was to develop a widely applicable method to tag L. monocytogenes strains, with a particular emphasis on the development of multiple strain competitive index assays.
resultsWe have constructed a new site-specific integrative vector, pIMC, based on pPL2, for the selection of L. monocytogenes from complex samples. The pIMC vector was further modified through the incorporation of IPTG inducible markers (antibiotic and phenotypic) to produce a suite of four vectors which allowed the discrimination of multiple strains from a single sample. We were able to perform murine infection studies with up to four EGDe isolates within a single mouse and showed that the tags did not impact upon growth rate or virulence. The system also allowed the identification of subtle differences in virulence between strains of L. monocytogenes commonly used in laboratory studies.
conclusionThis study has developed a competitive index assay that can be broadly applied to all L. monocytogenes strains. Improved statistical robustness of the data was observed, resulting in fewer mice being required for virulence assays. The competitive index assays provide a powerful method to analyse the virulence or fitness of L. monocytogenes in complex biological samples.
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