ArticleMolecular microbiology2008
Site-specific receptor methylation of FrzCD in Myxococcus xanthus is controlled by a tetra-trico peptide repeat (TPR) containing regulatory domain of the FrzF methyltransferase.
Article in Molecular microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Milestones in the development ofJournal of bacteriology · 2025Review
- Article
- Identification of two different chemosensory pathways in representatives of the genus Halomonas.BMC genomics · 2018Article
- Dual biochemical oscillators may control cellular reversals in Myxococcus xanthus.Biophysical journal · 2014Article
- Internal sense of direction: sensing and signaling from cytoplasmic chemoreceptors.Microbiology and molecular biology reviews : MMBR · 2014Review
- Cell division resets polarity and motility for the bacterium Myxococcus xanthus.Journal of bacteriology · 2014Article
- Phosphorylation-dependent localization of the response regulator FrzZ signals cell reversals in Myxococcus xanthus.Molecular microbiology · 2013Article
- Article
- Uncovering the mystery of gliding motility in the myxobacteria.Annual review of genetics · 2011Review
- Gliding motility revisited: how do the myxobacteria move without flagella?Microbiology and molecular biology reviews : MMBR · 2010Review
- A multi-protein complex from Myxococcus xanthus required for bacterial gliding motility.Molecular microbiology · 2010Article
- Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis.Infection and immunity · 2010Article
- AglZ regulates adventurous (A-) motility in Myxococcus xanthus through its interaction with the cytoplasmic receptor, FrzCD.Molecular microbiology · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Myxococcus xanthus is a gliding bacterium with a complex life cycle that includes swarming, predation and fruiting body formation. Directed movements in M. xanthus are regulated by the Frz chemosensory system, which controls cell reversals. The Frz pathway requires the activity of FrzCD, a cytoplasmic methyl-accepting chemotaxis protein, and FrzF, a methyltransferase (CheR) containing an additional domain with three tetra trico-peptide repeats (TPRs). To investigate the role of the TPRs in FrzCD methylation, we used full-length FrzF and FrzF lacking its TPRs (FrzF(CheR)) to methylate FrzCD in vitro. FrzF methylated FrzCD on a single residue, E182, while FrzF(CheR) methylated FrzCD on three residues, E168, E175 and E182, indicating that the TPRs regulate site-specific methylation. E168 and E182 were predicted consensus methylation sites, but E175 is methylated on an HE pair. To determine the roles of these sites in vivo, we substituted each methylatable glutamate with either an aspartate or an alanine residue and determined the impact of the point mutants on single cell reversals, swarming and fruiting body formation. Single, double and triple methylation site mutants revealed that each site played a unique role in M. xanthus behaviour and that the pattern of receptor methylation determined receptor activity. This work also shows that methylation can both activate and inactivate the receptor.
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