ArticleBiotechnology and bioengineering2008
The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells.
Article in Biotechnology and bioengineering, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Transcriptomic Insights Into Serum-Free Medium Adaptation and Temperature Reduction in Chinese Hamster Ovary Cell Cultures.Biotechnology journal · 2025Article
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- Human extracellular matrix (ECM)-like collagen and its bioactivity.Regenerative biomaterials · 2024Article
- Increase recombinant antibody yields through optimizing vector design and production process in CHO cells.Applied microbiology and biotechnology · 2022Article
- Modified secreted alkaline phosphatase as an improved reporter protein for N-glycosylation analysis.PloS one · 2021Article
- Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach.Frontiers in bioengineering and biotechnology · 2021Article
- Knockout of the caspase 8-associated protein 2 gene improves recombinant protein expression in HEK293 cells through up-regulation of the cyclin-dependent kinase inhibitor 2A gene.Biotechnology and bioengineering · 2021Article
- Glycosylation Heterogeneity of Hyperglycosylated Recombinant Human Interferon-β (rhIFN-β).ACS omega · 2020Article
- Glycosylation of Zika Virus is Important in Host-Virus Interaction and Pathogenic Potential.International journal of molecular sciences · 2019Article
- Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteins.Molecular human reproduction · 2018Article
- A protein chimera strategy supports production of a model "difficult-to-express" recombinant target.FEBS letters · 2018Article
- Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation.Biotechnology and bioengineering · 2018Article
- Modulation of Interleukin-12 activity in the presence of heparin.Scientific reports · 2017Article
- Model-based investigation of intracellular processes determining antibody Fc-glycosylation under mild hypothermia.Biotechnology and bioengineering · 2017Article
- IMAC capture of recombinant protein from unclarified mammalian cell feed streams.Biotechnology and bioengineering · 2016Article
- Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures.Biotechnology and bioengineering · 2015Article
- Comparative investigation of the use of various commercial microcarriers as a substrate for culturing mammalian cells.In vitro cellular & developmental biology. Animal · 2014Article
- Differential effect of culture temperature and specific growth rate on CHO cell behavior in chemostat culture.PloS one · 2014Article
- The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody.SpringerPlus · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The effects of different culture conditions, suspension and microcarrier culture and temperature reduction on the structures of N-linked glycans attached to secreted human placental alkaline phosphatase (SEAP) were investigated for CHO cells grown in a controlled bioreactor. Both mass spectrometry and anion-exchange chromatography were used to probe the N-linked glycan structures and distribution. Complex-type glycans were the dominant structures with small amounts of high mannose glycans observed in suspension and reduced temperature cultures. Biantennary glycans were the most common structures detected by mass spectrometry, but triantennary and tetraantennary forms were also detected. The amount of sialic acid present was relatively low, approximately 0.4 mol sialic acid/mol SEAP for suspension cultures. Microcarrier cultures exhibited a decrease in productivity compared with suspension culture due to a decrease in both maximum viable cell density (15-20%) and specific productivity (30-50%). In contrast, a biphasic suspension culture in which the temperature was reduced at the beginning of the stationary phase from 37 to 33 degrees C, showed a 7% increase in maximum viable cell density, a 62% increase in integrated viable cell density, and a 133% increase in specific productivity, leading to greater than threefold increase in total productivity. Both microcarrier and reduced temperature cultures showed increased sialylation and decreased fucosylation when compared to suspension culture. Our results highlight the importance of glycoform analysis after process modification as even subtle changes (e.g., changing from one microcarrier to another) may affect glycan distributions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.