ReviewHistochemistry and cell biology2008
Developments in cell biology for quantitative immunoelectron microscopy based on thin sections: a review.
Review in Histochemistry and cell biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
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- Spreading of Alpha Synuclein from Glioblastoma Cells towards Astrocytes Correlates with Stem-like Properties.Cancers · 2022Article
- Occurrence of Total and Proteinase K-Resistant Alpha-Synuclein in Glioblastoma Cells Depends on mTOR Activity.Cancers · 2022Article
- Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells.Journal of pain research · 2020Article
- Imaging the Cardiac Extracellular Matrix.Advances in experimental medicine and biology · 2018Review
- Expression and distribution of three transient receptor potential vanilloid(TRPV) channel proteins in human odontoblast-like cells.Journal of molecular histology · 2017Article
- CD44: a novel synaptic cell adhesion molecule regulating structural and functional plasticity of dendritic spines.Molecular biology of the cell · 2016Article
- Using electron microscopes to look into the lung.Histochemistry and cell biology · 2016Review
- From gross anatomy to the nanomorphome: stereological tools provide a paradigm for advancing research in quantitative morphomics.Journal of anatomy · 2015Article
- Effects of ovariectomy on the secretory apparatus in the right atrial cardiomyocytes of middle-aged mice.Clinics (Sao Paulo, Brazil) · 2014Article
- Mapping the distributions and quantifying the labelling intensities of cell compartments by immunoelectron microscopy: progress towards a coherent set of methods.Journal of anatomy · 2011Review
- Multiple-labelling immunoEM using different sizes of colloidal gold: alternative approaches to test for differential distribution and colocalization in subcellular structures.Histochemistry and cell biology · 2011Article
- Localization of carboxyl ester lipase in human pituitary gland and pituitary adenomas.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Quantitative assessment of specificity in immunoelectron microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Article
- Extending the knowledge in histochemistry and cell biology.Histochemistry and cell biology · 2010Review
- A stereological approach for estimation of cellular immunogold labeling and its spatial distribution in oriented sections using the rotator.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- A stereological perspective on placental morphology in normal and complicated pregnancies.Journal of anatomy · 2009Review
- State-of-the-art technologies, current opinions and developments, and novel findings: news from the field of histochemistry and cell biology.Histochemistry and cell biology · 2008Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative immunoelectron microscopy uses ultrathin sections and gold particle labelling to determine distributions of molecules across cell compartments. Here, we review a portfolio of new methods for comparing labelling distributions between different compartments in one study group (method 1) and between the same compartments in two or more groups (method 2). Specimen samples are selected unbiasedly and then observed and expected distributions of gold particles are estimated and compared by appropriate statistical procedures. The methods can be used to analyse gold label distributed between volume-occupying (organelle) and surface-occupying (membrane) compartments, but in method 1, membranes must be treated as organelles. With method 1, gold counts are combined with stereological estimators of compartment size to determine labelling density (LD). For volume-occupiers, LD can be expressed simply as golds per test point and, for surface-occupiers, as golds per test line intersection. Expected distributions are generated by randomly assigning gold particles to compartments and expressing observed/expected counts as a relative labelling index (RLI). Preferentially-labelled compartments are identified from their RLI values and by Chi-squared analysis of observed and expected distributions. For method 2, the raw gold particle counts distributed between compartments are simply compared across groups by contingency table and Chi-squared analysis. This identifies the main compartments responsible for the differences between group distributions. Finally, we discuss labelling efficiency (the number of gold particles per target molecule) and describe how it can be estimated for volume- or surface-occupiers by combining stereological data with biochemical determinations.
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Registered trials
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