ArticlePlant cell reports2008
Direct transformation of the liverwort Marchantia polymorpha L. by particle bombardment using immature thalli developing from spores.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed.
- Ancient peptide-redox signalling underlies sperm motility in Marchantia.Nature plants · 2026Article
- Conservation and divergence of UVR8 photoreceptor-mediated UV-B signaling in Marchantia polymorpha.Plant physiology · 2026Article
- The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha.Journal of plant research · 2025Article
- Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation.The Plant journal : for cell and molecular biology · 2025Article
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- Reproducibly oriented cell divisions pattern the prothallus to set up dorsoventrality and de novo meristem formation in Marchantia polymorpha.Current biology : CB · 2024Article
- MpMLO1 controls sperm discharge in liverwort.Nature plants · 2024Article
- Thermospermine Is an Evolutionarily Ancestral Phytohormone Required for Organ Development and Stress Responses in Marchantia Polymorpha.Plant & cell physiology · 2024Article
- Biosynthesis of gibberellin-related compounds modulates far-red light responses in the liverwort Marchantia polymorpha.The Plant cell · 2023Article
- Auxin signaling is essential for organogenesis but not for cell survival in the liverwort Marchantia polymorpha.The Plant cell · 2023Article
- Circadian clock does not play an essential role in daylength measurement for growth-phase transition inFrontiers in plant science · 2023Article
- Selection of a histidine auxotrophicPlant biotechnology (Tokyo, Japan) · 2022Article
- The renaissance and enlightenment of Marchantia as a model system.The Plant cell · 2022Article
- Image-Based Analysis Revealing the Molecular Mechanism of Peroxisome Dynamics in Plants.Frontiers in cell and developmental biology · 2022Review
- Regulation of gametangia and gametangiophore initiation in the liverwort Marchantia polymorpha.Plant reproduction · 2021Review
- Chloroplast relocation movement in the liverwort Apopellia endiviifolia.Physiologia plantarum · 2021Article
- An Agrobacterium-mediated stable transformation technique for the hornwort model Anthoceros agrestis.The New phytologist · 2021Article
- Far-Red Light-InducedFrontiers in plant science · 2021Article
- Design principles of a minimal auxin response system.Nature plants · 2020Article
- A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein-Protein Interactions and Cell Morphology inFrontiers in plant science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The liverwort, Marchantia polymorpha L., belongs to a group of basal land plants and is an emerging model for plant biology. We established a procedure to prepare sporangia of M. polymorpha under laboratory conditions by promoting its transition to reproductive development by far-red light irradiation. Here we report an improved direct transformation system of M. polymorpha using immature thalli developing from spores. Hygromycin-resistant transformants were obtained on selective media by transformation with a plasmid carrying the hygromycin-phosphotransferase gene (hpt) conferring hygromycin resistance in 4 weeks. The aminoglycoside-3''-adenyltransferase gene (aadA) conferring spectinomycin resistance was also successfully used as an additional selectable marker for nuclear transformation of M. polymorpha. The availability of the aadA gene in addition to the hpt gene should make M. polymorpha a versatile host for genetic manipulation. DNA gel-blot analyses indicated that transformed thalli carried a variable number of copies of the transgene integrated into the genome. Although the previous system using thalli grown from gemmae required a two-step selection in liquid and solid media for 8 weeks, the system reported here using thalli developing from spores allows generation of transformants in half the time by direct selection on solid media, facilitating genetic analyses in this model plant.
Indexed as
Identifiers
18553085What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.