Evidence map›Paper›PMID 18553067›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008

The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley.

Anke Lehmensiek, Mark W Sutherland, Raymond B McNamara

Abstract read
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In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 87 citations in OpenAlex.

  1. Article
  2. The use of a candidate gene approach to studyFrontiers in plant science · 2023
    Article
  3. Article
  4. Article
  5. Identification of a molecular marker tightly linked to bacterial wilt resistance in tomato by genome-wide SNP analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018
    Article
  6. Article
  7. Article
  8. Article
  9. Assessment for Melting Temperature Measurement of Nucleic Acid by HRM.Journal of analytical methods in chemistry · 2016
    Article
  10. Article
  11. Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. SNP markers and their impact on plant breeding.International journal of plant genomics · 2012
    Article
  17. Article
  18. Article
  19. Genetic map of artichoke × wild cardoon: toward a consensus map for Cynara cardunculus.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Anke LehmensiekCentre for Systems Biology, University of Southern Queensland, Toowoomba QLD 4350, Australia. lehmensi@usq.edu.au
Mark W Sutherland
Raymond B McNamara
University of Southern Queensland · AUArt Research Centre of the Slovak Academy of Sciences · SK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using an established genetic map, a single gene conditioning covered smut resistance, Ruh.7H, was mapped to the telomere region of chromosome 7HS in an Alexis/Sloop doubled haploid barley population. The closest marker to Ruh.7H, abg704 was 7.5 cM away. Thirteen loci on the distal end of 7HS with potential to contain single nucleotide polymorphisms (SNPs) were identified by applying a comparative genomics approach using rice sequence data. Of these, one locus produced polymorphic co-dominant bands of different size while two further loci contained SNPs that were identified using the recently developed high resolution melting (HRM) technique. Two of these markers flanked Ruh.7H with the proximal marker located 3.8 cM and the distal marker 2.7 cM away. This is the first report on the application of the HRM technique to SNP detection and to rapid scoring of known cleaved amplified polymorphic sequence (CAPS) markers in plants. This simple, precise post-PCR technique should find widespread use in the fine-mapping of genetic regions of interest in complex cereal and other plant genomes.

Indexed as

Polymorphism, Single NucleotideChromosome MappingChromosomes, PlantExpressed Sequence TagsGenes, PlantGenetic LinkageGenetic MarkersHordeumImmunity, InnatePhenotypePlant DiseasesUstilagoGenetic Markers

Identifiers

PMID18553067
OpenAlexW1981671745

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.