ArticleThe Plant cell2008
Arabidopsis DDB1-CUL4 ASSOCIATED FACTOR1 forms a nuclear E3 ubiquitin ligase with DDB1 and CUL4 that is involved in multiple plant developmental processes.
Article in The Plant cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed, 165 citations in OpenAlex.
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- Genome-wide identification and characterization of the Groucho/Tup1-like corepressor family identifies a potential role in the epigenetic regulation of abiotic stress responses in soybean.Frontiers in plant science · 2026Article
- Striking convergent selection history of wheat and barley and its potential for breeding.Nature plants · 2025Article
- The E3 ubiquitin ligase SlATL2 suppresses tomato immunity by promoting SlCSN5a degradation duringHorticulture research · 2025Article
- The RAE1-STOP1 module regulates ABA sensitivity in early seedlings of Arabidopsis.BMC plant biology · 2025Article
- Genomic selection and genetic architecture of agronomic traits during modern flowering Chinese cabbage breeding.Horticulture research · 2025Article
- Identification of mitogen-activated protein kinases substrates inPlant signaling & behavior · 2024Article
- Nuclear lamina component KAKU4 regulates chromatin states and transcriptional regulation in the Arabidopsis genome.BMC biology · 2024Article
- E3 ligases MAC3A and MAC3B ubiquitinate UBIQUITIN-SPECIFIC PROTEASE14 to regulate organ size in Arabidopsis.Plant physiology · 2024Article
- APRF1 Interactome Reveals HSP90 as a New Player in the Complex That Epigenetically Regulates Flowering Time inInternational journal of molecular sciences · 2024Article
- The Abundant and Unique Transcripts and Alternative Splicing of the Artificially Autododecaploid London Plane (International journal of molecular sciences · 2023Article
- Article
- The translational landscape of bread wheat during grain development.The Plant cell · 2023Article
- ABA signaling prevents phosphodegradation of the SR45 splicing factor to alleviate inhibition of early seedling development in Arabidopsis.Plant communications · 2023Article
- The noncoding RNA HIDDEN TREASURE 1 promotes phytochrome B-dependent seed germination by repressing abscisic acid biosynthesis.The Plant cell · 2023Article
- Genome-Wide Identification of WD40 Proteins inGenes · 2023Article
- Genome-wide analysis of WD40 protein family and functional characterization ofFrontiers in plant science · 2023Article
- A single helix repression domain is functional across diverse eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Comparative Transcriptome Analysis Reveals the Molecular Mechanisms That Underlie Somatic Embryogenesis inInternational journal of molecular sciences · 2022Article
24 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human DDB1-CUL4 ASSOCIATED FACTOR (DCAF) proteins have been reported to interact directly with UV-DAMAGED DNA BINDING PROTEIN1 (DDB1) through the WDxR motif in their WD40 domain and function as substrate-recognition receptors for CULLIN4-based E3 ubiquitin ligases. Here, we identified and characterized a homolog of human DCAF1/VprBP in Arabidopsis thaliana. Yeast two-hybrid analysis demonstrated the physical interaction between DCAF1 and DDB1 from Arabidopsis, which is likely mediated via the WD40 domain of DCAF1 that contains two WDxR motifs. Moreover, coimmunoprecipitation assays showed that DCAF1 associates with DDB1, RELATED TO UBIQUITIN-modified CUL4, and the COP9 signalosome in vivo but not with CULLIN-ASSOCIATED and NEDDYLATION-DISSOCIATED1, CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1), or the COP10-DET1-DDB1 complex, supporting the existence of a distinct Arabidopsis CUL4 E3 ubiquitin ligase, the CUL4-DDB1-DCAF1 complex. Transient expression of fluorescently tagged DCAF1, DDB1, and CUL4 in onion epidermal cells showed their colocalization in the nucleus, consistent with the notion that the CUL4-DDB1-DCAF1 complex functions as a nuclear E3 ubiquitin ligase. Genetic and phenotypic analysis of two T-DNA insertion mutants of DCAF1 showed that embryonic development of the dcaf1 homozygote is arrested at the globular stage, indicating that DCAF1 is essential for plant embryogenesis. Reducing the levels of DCAF1 leads to diverse developmental defects, implying that DCAF1 might be involved in multiple developmental pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.