ArticleTransgenic research2009
Genetic transformation of the sugar beet plastome.
Article in Transgenic research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 71 citations in OpenAlex.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- CRISPR/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Frontiers in genome editing · 2026Article
- Stable plastid transformation in kiwifruit (aBIOTECH · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- Engineering the plastid and mitochondrial genomes of flowering plants.Nature plants · 2022Review
- Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.Plant biotechnology journal · 2019Article
- Paternal inheritance of plastid-encoded transgenes inBiotechnology reports (Amsterdam, Netherlands) · 2017Article
- A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha.Plant & cell physiology · 2016Article
- The Engineered Chloroplast Genome Just Got Smarter.Trends in plant science · 2015Review
- Low frequency paternal transmission of plastid genes in Brassicaceae.Transgenic research · 2015Article
- Plastid genomics in horticultural species: importance and applications for plant population genetics, evolution, and biotechnology.Frontiers in plant science · 2015Review
- Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Plastid biotechnology for crop production: present status and future perspectives.Plant molecular biology · 2011Article
- Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- High efficiency plastid transformation in potato and regulation of transgene expression in leaves and tubers by alternative 5' and 3' regulatory sequences.Transgenic research · 2011Article
- Plant plastid engineering.Current genomics · 2010Article
- Pollen dispersal in sugar beet production fields.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is very important for the application of chloroplast engineering to extend the range of species in which this technology can be achieved. Here, we describe the development of a chloroplast transformation system for the sugar beet (Beta vulgaris L. ssp. vulgaris, Sugar Beet Group) by biolistic bombardment of leaf petioles. Homoplasmic plastid-transformed plants of breeding line Z025 were obtained. Transformation was achieved using a vector that targets genes to the rrn16/rps12 intergenic region of the sugar beet plastome, employing the aadA gene as a selectable marker against spectinomycin and the gfp gene for visual screening of plastid transformants. gfp gene transcription and protein expression were shown in transplastomic plants. Detection of GFP in Comassie blue-stained gels suggested high GFP levels. Microscopy revealed GFP fluorescence within the chloroplasts. Our results demonstrate the feasibility of engineering the sugar beet chloroplast genome; this technology provides new opportunities for the genetic improvement of this crop and for social acceptance of genetically modified sugar beet plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.