ArticlePLoS genetics2008
SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex.
Article in PLoS genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Rice and shine: a review of miRNA-mediated responses to abiotic stress inFrontiers in plant science · 2026Review
- Properties of Plant Virus Protein Encoded by the 5'-Proximal Gene of Tetra-Cistron Movement Block.International journal of molecular sciences · 2023Article
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Silencing ofPlant signaling & behavior · 2022Article
- Recent advances in the regulation of plant miRNA biogenesis.RNA biology · 2021Review
- Non-Coding RNAs in Response to Drought Stress.International journal of molecular sciences · 2021Review
- Interplay between miRNAs and lncRNAs: Mode of action and biological roles in plant development and stress adaptation.Computational and structural biotechnology journal · 2021Review
- Plant microRNAs: Biogenesis, Homeostasis, and Degradation.Frontiers in plant science · 2019Review
- Sex Determination inG3 (Bethesda, Md.) · 2018Article
- Mechanisms of microRNA-mediated gene regulation in unicellular model algaBiotechnology for biofuels · 2018Review
- Intron Lariat RNA Inhibits MicroRNA Biogenesis by Sequestering the Dicing Complex in Arabidopsis.PLoS genetics · 2016Article
- microRNA biogenesis, degradation and activity in plants.Cellular and molecular life sciences : CMLS · 2015Review
- Article
- The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana.Nucleic acids research · 2014Article
- Protein-coding cis-natural antisense transcripts have high and broad expression in Arabidopsis.Plant physiology · 2013Article
- Post-transcriptional control of miRNA abundance in Arabidopsis.Plant signaling & behavior · 2012Review
- Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection.The Plant journal : for cell and molecular biology · 2012Article
- microRNA biogenesis and turnover in plants.Cold Spring Harbor symposia on quantitative biology · 2012Review
- Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels in Arabidopsis.RNA (New York, N.Y.) · 2011Article
- Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proper temporal and spatial expression of genes during plant development is governed, in part, by the regulatory activities of various types of small RNAs produced by the different RNAi pathways. Here we report that transgenic Arabidopsis plants constitutively expressing the rapeseed SB1 SINE retroposon exhibit developmental defects resembling those observed in some RNAi mutants. We show that SB1 RNA interacts with HYL1 (DRB1), a double-stranded RNA-binding protein (dsRBP) that associates with the Dicer homologue DCL1 to produce microRNAs. RNase V1 protection assays mapped the binding site of HYL1 to a SB1 region that mimics the hairpin structure of microRNA precursors. We also show that HYL1, upon binding to RNA substrates, induces conformational changes that force single-stranded RNA regions to adopt a structured helix-like conformation. Xenopus laevis ADAR1, but not Arabidopsis DRB4, binds SB1 RNA in the same region as HYL1, suggesting that SINE RNAs bind only a subset of dsRBPs. Consistently, DCL4-DRB4-dependent miRNA accumulation was unchanged in SB1 transgenic Arabidopsis, whereas DCL1-HYL1-dependent miRNA and DCL1-HYL1-DCL4-DRB4-dependent tasiRNA accumulation was decreased. We propose that SINE RNA can modulate the activity of the RNAi pathways in plants and possibly in other eukaryotes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.