ArticleProceedings of the National Academy of Sciences of the United States of America2008
DEAF-1 regulates immunity gene expression in Drosophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Identification and Screening of LITAF Family Key Genes Responsive to Plant Secondary Metabolites inBiology · 2026Article
- Weighted Gene Co-Expression Network Analysis Based on Stimulation by Lipopolysaccharides and Polyinosinic:polycytidylic Acid Provides a Core Set of Genes for Understanding Hemolymph Immune Response Mechanisms ofAnimals : an open access journal from MDPI · 2023Article
- Zinc finger myeloid Nervy DEAF-1 type (ZMYND) domain containing proteins exert molecular interactions to implicate in carcinogenesis.Discover oncology · 2022Review
- A large-scale transgenic RNAi screen identifies transcription factors that modulate myofiber size in Drosophila.PLoS genetics · 2021Article
- Regulatory regions in natural transposable element insertions drive interindividual differences in response to immune challenges in Drosophila.Genome biology · 2021Article
- TheInternational journal of molecular sciences · 2021Article
- The Speckled Protein (SP) Family: Immunity's Chromatin Readers.Trends in immunology · 2020Review
- Diverse families of transposable elements affect the transcriptional regulation of stress-response genes in Drosophila melanogaster.Nucleic acids research · 2019Article
- Article
- A multi-faceted approach testing the effects of previous bacterial exposure on resistance and tolerance.The Journal of animal ecology · 2019Article
- Chromatin changes in Anopheles gambiae induced by Plasmodium falciparum infection.Epigenetics & chromatin · 2019Article
- DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori.Epigenetics & chromatin · 2018Article
- Article
- Multiple Independent Retroelement Insertions in the Promoter of a Stress Response Gene Have Variable Molecular and Functional Effects in Drosophila.PLoS genetics · 2016Article
- Engineering of a high lipid producing Yarrowia lipolytica strain.Biotechnology for biofuels · 2016Article
- Type 1 diabetes in mice and men: gene expression profiling to investigate disease pathogenesis.Immunologic research · 2014Review
- MEF2 is an in vivo immune-metabolic switch.Cell · 2013Article
- DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA.The Journal of biological chemistry · 2013Article
- The identification of trans-acting factors that regulate the expression of GDF5 via the osteoarthritis susceptibility SNP rs143383.PLoS genetics · 2013Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Immunity genes are activated in the Drosophila fat body by Rel and GATA transcription factors. Here, we present evidence that an additional regulatory factor, deformed epidermal autoregulatory factor-1 (DEAF-1), also contributes to the immune response and is specifically important for the induction of two genes encoding antimicrobial peptides, Metchnikowin (Mtk) and Drosomycin (Drs). The systematic mutagenesis of a minimal Mtk 5' enhancer identified a sequence motif essential for both a response to LPS preparations in S2 cells and activation in the larval fat body in response to bacterial infection. Using affinity chromatography coupled to multidimensional protein identification technology (MudPIT), we identified DEAF-1 as a candidate regulator. DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. SELEX assays and footprinting data indicate that DEAF-1 binds to and activates Mtk and Drs regulatory DNAs via a TTCGGBT motif. The insertion of this motif into the Diptericin (Dpt) regulatory region confers DEAF-1 responsiveness to this normally DEAF-1-independent enhancer. The coexpression of DEAF-1 with Dorsal, Dif, and Relish results in the synergistic activation of transcription. We propose that DEAF-1 is a regulator of Drosophila immunity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.