ArticleJournal of clinical microbiology2008
Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 3 of them syntheses that pooled it.
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Who cites it
22 citing papers in PubMed, 3 syntheses or guidelines pooled it, 72 citations in OpenAlex.
- Molecular assays for the diagnosis of sepsis in neonates: a diagnostic test accuracy review.The Cochrane database of systematic reviews · 2025Pooled it
- Clinical Utility of Molecular Tests for Guiding Therapeutic Decisions in Bloodstream Staphylococcal Infections: A Meta-Analysis.Frontiers in pediatrics · 2021Pooled it
- Molecular assays for the diagnosis of sepsis in neonates.The Cochrane database of systematic reviews · 2017Pooled it
- Review
- Article
- Biomimetic Magnetic Particles for the Removal of Gram-Positive Bacteria and Lipoteichoic Acid.Pharmaceutics · 2022Article
- MetaFunPrimer: an Environment-Specific, High-Throughput Primer Design Tool for Improved Quantification of Target Genes.mSystems · 2021Article
- Ultrasensitive digital quantification of cytokines and bacteria predicts septic shock outcomes.Nature communications · 2020Article
- Droplet digital polymerase chain reaction for rapid broad-spectrum detection of bloodstream infections.Microbial biotechnology · 2020Article
- Assessment and comparison of bacterial load levels determined by quantitative amplifications in blood culture-positive and negative neonatal sepsis.Revista do Instituto de Medicina Tropical de Sao Paulo · 2018Article
- Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis.Critical care (London, England) · 2018Article
- Diagnostics for neonatal sepsis: current approaches and future directions.Pediatric research · 2017Review
- Impact of round-the-clock CSF Gram stain on empirical therapy for suspected central nervous system infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Rapid and Sensitive Identification of Bacterial Infection and Bacteria Gram Types in Pleural Fluid of Children.Global pediatric health · 2015Article
- Article
- Diagnosis by real-time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections.BMC infectious diseases · 2013Article
- How can the microbiologist help in diagnosing neonatal sepsis?International journal of pediatrics · 2012Article
- Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis.Journal of pathogens · 2012Article
- Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor.Journal of the American Chemical Society · 2011Article
- Biomarkers for late-onset neonatal sepsis: cytokines and beyond.Clinics in perinatology · 2010Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sepsis is a serious disease with high mortality in newborns. It is very important to have a convenient and accurate method for pathogenic diagnosis of neonatal sepsis. We developed a method of simultaneous detection and Gram classification of clinically relevant bacterial pathogens causing sepsis directly from blood samples with Gram stain-specific-probe-based real-time PCR (GSPBRT-PCR). With GSPBRT-PCR, 53 clinically important strains representing 25 gram-positive and 28 gram-negative bacterial species were identified correctly with the corresponding Gram probe. The limits of the GSPBRT-PCR assay in serial dilutions of the bacteria revealed that Staphylococcus aureus could be detected at concentrations of 3 CFU per PCR and Escherichia coli at concentrations as low as 1 CFU per PCR. The GSPBRT-PCR assay was further evaluated on 600 blood specimens from patients with suspicion of neonatal sepsis and compared to the results obtained from blood cultures. The positive rate of the GSPBRT-PCR array was 50/600 (8.33%), significantly higher than that of blood culture (34/600; 5.67%) (P = 0.00003). When blood culture was used as a control, the sensitivity of GSPBRT-PCR was 100%, the specificity was 97.17%, and the index of accurate diagnosis was 0.972. This study suggests that GSPBRT-PCR is very useful for the rapid and accurate diagnosis of bacterial infection and that it can have an important impact on the current inappropriate and unnecessary use of antibiotics in the treatment of newborns.
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Registered trials
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