ArticleClinical and vaccine immunology : CVI2008
Diagnosis of bovine paratuberculosis by a novel enzyme-linked immunosorbent assay based on early secreted antigens of Mycobacterium avium subsp. paratuberculosis.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 49 citations in OpenAlex.
- Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species.Veterinary medicine international · 2026Article
- Vaccination inducing durable and robust antigen-specific Th1/Th17 immune responses contributes to prophylactic protection againstVirulence · 2022Article
- An update on Mycobacterium avium subspecies paratuberculosis antigens and their role in the diagnosis of Johne's disease.World journal of microbiology & biotechnology · 2019Review
- PCR Inhibition of a Quantitative PCR for Detection ofFrontiers in microbiology · 2017Article
- Composition and Potency Characterization of Mycobacterium avium subsp. paratuberculosis Purified Protein Derivatives.PloS one · 2016Article
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- Novel secreted antigens of Mycobacterium paratuberculosis as serodiagnostic biomarkers for Johne's disease in cattle.Clinical and vaccine immunology : CVI · 2013Article
- Flow cytometric detection of Mycobacterium avium subsp. paratuberculosis-specific antibodies in experimentally infected and naturally exposed calves.Clinical and vaccine immunology : CVI · 2013Article
- Mycobacterium avium subspecies paratuberculosis: an insidious problem for the ruminant industry.Tropical animal health and production · 2013Review
- Search for Mycobacterium avium Subspecies paratuberculosis Antigens for the Diagnosis of Paratuberculosis.Veterinary medicine international · 2012Article
- Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis.Veterinary medicine international · 2012Article
- Comparative analysis of immune responses to Mycobacterium abscessus infection and its antigens in two murine models.Journal of microbiology (Seoul, Korea) · 2009Article
- Rapid mycobacterial liquid culture-screening method for Mycobacterium avium complex based on secreted antigen-capture enzyme-linked immunosorbent assay.Clinical and vaccine immunology : CVI · 2009Article
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Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that protein antigens of serodiagnostic potential were more abundant in culture filtrates than cellular extracts from liquid cultures of Mycobacterium avium subsp. paratuberculosis (D. Cho and M. T. Collins, Clin. Vaccine Immunol. 13:1155-1161, 2006). Based on this observation, a novel enzyme-linked immunosorbent assay (ELISA) using antigens secreted by young (early- to mid-log-phase) cultures of M. avium subsp. paratuberculosis JTC303 (a low-passage isolate originating from the ileum of a Holstein bull) in mycobactin-supplemented Watson-Reid medium (pH 6.0) was developed and evaluated using a previously described panel of bovine sera (M. T. Collins et al., Clin. Diagn. Lab. Immunol. 12:685-692, 2005) that included 444 paratuberculosis cases and 412 controls. The new assay, called JTC-ELISA, had a significantly higher diagnostic sensitivity and an equivalent specificity compared to those of five commercial paratuberculosis ELISA kits. By receiver-operating characteristic analysis, the JTC-ELISA had the highest area under the curve of the six assays evaluated. The JTC-ELISA was particularly sensitive at detecting low-level fecal shedders of Mavium subsp. paratuberculosis (40%; the sensitivity of the commercial kits was 20%). The JTC-ELISA works effectively on both serum and milk samples for the detection of cattle with subclinical M. avium subsp. paratuberculosis infections, providing a cost-effective diagnostic tool to support paratuberculosis control programs in cattle herds.
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