ArticleDevelopment (Cambridge, England)2008
Convergence and extension at gastrulation require a myosin IIB-dependent cortical actin network.
Article in Development (Cambridge, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
103 citing papers in PubMed, 174 citations in OpenAlex.
- Quantifying convergent extension: Shih and Keller's quintessential work in developmental cell biology.Developmental biology · 2025Article
- PCP-dependent polarized mechanics in the cortex of individual cells during convergent extension.Developmental biology · 2025Article
- PCP and Septins govern the polarized organization of the actin cytoskeleton during convergent extension.Current biology : CB · 2024Article
- Actomyosin pulsing rescues embryonic tissue folding from disruption by myosin fluctuations.Research square · 2023Article
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- Engineered kinases as a tool for phosphorylation of selected targets in vivo.The Journal of cell biology · 2022Article
- Forces in stem cells and cancer stem cells.Cells & development · 2022Review
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- Revealing epithelial morphogenetic mechanisms through live imaging.Current opinion in genetics & development · 2022Review
- Generation of anisotropic strain dysregulates wild-type cell division at the interface between host and oncogenic tissue.Current biology : CB · 2021Article
- Twinfilin1 controls lamellipodial protrusive activity and actin turnover during vertebrate gastrulation.Journal of cell science · 2021Article
- Mechanical feedback and robustness of apical constrictions in Drosophila embryo ventral furrow formation.PLoS computational biology · 2021Article
- A temporally resolved transcriptome for developing "Keller" explants of the Xenopus laevis dorsal marginal zone.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- Programmed and self-organized flow of information during morphogenesis.Nature reviews. Molecular cell biology · 2021Review
- Cellular and Supracellular Planar Polarity: A Multiscale Cue to Elongate theFrontiers in cell and developmental biology · 2021Review
- Cell intercalation in a simple epithelium.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Cellular processes driving gastrulation in the avian embryo.Mechanisms of development · 2020Review
- Convergent extension in mammalian morphogenesis.Seminars in cell & developmental biology · 2020Review
- Tissue mechanics drives regeneration of a mucociliated epidermis on the surface of Xenopus embryonic aggregates.Nature communications · 2020Article
- Magnetic Micromanipulation forResearch (Washington, D.C.) · 2020Article
43 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Force-producing convergence (narrowing) and extension (lengthening) of tissues by active intercalation of cells along the axis of convergence play a major role in axial morphogenesis during embryo development in both vertebrates and invertebrates, and failure of these processes in human embryos leads to defects including spina bifida and anencephaly. Here we use Xenopus laevis, a system in which the polarized cell motility that drives this active cell intercalation has been related to the development of forces that close the blastopore and elongate the body axis, to examine the role of myosin IIB in convergence and extension. We find that myosin IIB is localized in the cortex of intercalating cells, and show by morpholino knockdown that this myosin isoform is essential for the maintenance of a stereotypical, cortical actin cytoskeleton as visualized with time-lapse fluorescent confocal microscopy. We show that this actin network consists of foci or nodes connected by cables and is polarized relative to the embryonic axis, preferentially cyclically shortening and lengthening parallel to the axis of cell polarization, elongation and intercalation, and also parallel to the axis of convergence forces during gastrulation. Depletion of MHC-B results in disruption of this polarized cytoskeleton, loss of the polarized protrusive activity characteristic of intercalating cells, eventual loss of cell-cell and cell-matrix adhesion, and dose-dependent failure of blastopore closure, arguably because of failure to develop convergence forces parallel to the myosin IIB-dependent dynamics of the actin cytoskeleton. These findings bridge the gap between a molecular-scale motor protein and tissue-scale embryonic morphogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.