ArticleJournal of virology2008
The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Heterologous avian system for quantitative analysis of Syncytin-1 interaction with ASCT2 receptor.Retrovirology · 2021Article
- Article
- Accumulation of long-term transcriptionally active integrated retroviral vectors in active promoters and enhancers.Nucleic acids research · 2017Article
- Impact of Different Promoters on Episomal Vectors Harbouring Characteristic Motifs of Matrix Attachment Regions.Scientific reports · 2016Article
- Impact of using different promoters and matrix attachment regions on recombinant protein expression level and stability in stably transfected CHO cells.Molecular biotechnology · 2015Article
- Alpharetroviral vectors: from a cancer-causing agent to a useful tool for human gene therapy.Viruses · 2014Review
- Virus-like attachment sites and plastic CpG islands:landmarks of diversity in plant Del retrotransposons.PloS one · 2014Article
- Role of DNA methylation in expression and transmission of porcine endogenous retroviruses.Journal of virology · 2013Article
- Retroviral DNA methylation and epigenetic repression are mediated by the antiviral host protein Daxx.Journal of virology · 2013Article
- Physiological regulation of transgene expression by a lentiviral vector containing the A2UCOE linked to a myeloid promoter.Gene therapy · 2012Article
- Transcriptional provirus silencing as a crosstalk of de novo DNA methylation and epigenomic features at the integration site.Nucleic acids research · 2012Article
- Retroviruses and retroelements in diseases and in gene therapy: 15 years later.Infectious agents and cancer · 2011Article
- Adeno-associated viral vector-mediated transgene expression is independent of DNA methylation in primate liver and skeletal muscle.PloS one · 2011Article
- Allele-specific DNA methylation: beyond imprinting.Human molecular genetics · 2010Review
- Human endogenous retroviral long terminal repeat sequences as cell type-specific promoters in retroviral vectors.Journal of virology · 2009Article
- Toward stable gene expression in CHO cells.BioengineeredArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unmethylated CpG islands are known to keep adjacent promoters transcriptionally active. In the CpG island adjacent to the adenosine phosphoribosyltransferase gene, the protection against transcriptional silencing can be attributed to the short CpG-rich core element containing Sp1 binding sites. We report here the insertion of this CpG island core element, IE, into the long terminal repeat of a retroviral vector derived from Rous sarcoma virus, which normally suffers from progressive transcriptional silencing in mammalian cells. IE insertion into a specific position between enhancer and promoter sequences led to efficient protection of the integrated vector from silencing and gradual CpG methylation in rodent and human cells. Individual cell clones with IE-modified reporter vectors display high levels of reporter expression for a sustained period and without substantial variegation in the cell culture. The presence of Sp1 binding sites is important for the protective effect of IE, but at least some part of the entire antisilencing capacity is maintained in IE with mutated Sp1 sites. We suggest that this strategy of antisilencing protection by the CpG island core element may prove generally useful in retroviral vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.