ArticleBMC microbiology2008
Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification.
Article in BMC microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 93 citations in OpenAlex.
- Isothermal nucleic acid amplification techniques: A comprehensive overview on their principle & applications as alternative to PCR.Practical laboratory medicine · 2026Review
- Development of a simple, rapid, and sensitive molecular diagnostic assay for cholera.PLoS neglected tropical diseases · 2023Article
- Utilization of Small RNA Genes to DistinguishThe American journal of tropical medicine and hygiene · 2019Article
- Development of uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification coupled with nanogold probe (UDG-LAMP-AuNP) for specific detection of Pseudomonas aeruginosa.Molecular medicine reports · 2018Article
- The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen.Molecular genetics, microbiology and virology : Molekulyarnaya genetika, mikrobiologiya i virusologiya · 2017Article
- Comparison of DOT-ELISA and Standard-ELISA for Detection of the Vibrio cholerae Toxin in Culture Supernatants of Bacteria Isolated from Human and Environmental Samples.Indian journal of microbiology · 2016Article
- An Innovative Method for Rapid Identification and Detection of Vibrio alginolyticus in Different Infection Models.Frontiers in microbiology · 2016Article
- Molecular inversion probe: a new tool for highly specific detection of plant pathogens.PloS one · 2014Article
- Alternative molecular tests for virological diagnosis.Molecular biotechnology · 2013Review
- Development of an immunochromatographic test strip for detection of cholera toxin.BioMed research international · 2013Article
- Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.Virology journal · 2012Article
- Development of a reverse transcription-loop-mediated isothermal amplification assay for detection of pandemic (H1N1) 2009 virus as a novel molecular method for diagnosis of pandemic influenza in resource-limited settings.Journal of clinical microbiology · 2010Article
- Development of a toxR-based loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus.BMC microbiology · 2010Article
- Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of Vibrio parahaemolyticus and related Vibrio species.Applied and environmental microbiology · 2010Article
- A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification.Virology journal · 2010Article
- Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases.Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2009Review
- Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples.Applied and environmental microbiology · 2009Article
- Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus.BMC microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio cholerae is widely acknowledged as one of the most important waterborne pathogen causing gastrointestinal disorders. Cholera toxin (CT) is a major virulence determinant of V. cholerae. Detection of CT-producing V. cholerae using conventional culture-, biochemical- and immunological-based assays is time-consuming and laborious, requiring more than three days. Thus, we developed a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the sensitive and rapid detection of cholera toxin (CT)-producing Vibrio cholerae.
resultsThe assay provided markedly more sensitive and rapid detection of CT-producing V. cholerae strains than conventional biochemical and PCR assays. The assay correctly identified 34 CT-producing V. cholerae strains, but did not detect 13 CT non-producing V. cholerae and 53 non-V. cholerae strains. Sensitivity of the LAMP assay for direct detection of CT-producing V. cholerae in spiked human feces was 7.8 x 102 CFU per g (1.4 CFU per reaction). The sensitivity of the LAMP assay was 10-fold more sensitive than that of the conventional PCR assay. The LAMP assay for detection of CT-producing V. cholerae required less than 35 min with a single colony on thiosulfate citrate bile salt sucrose (TCBS) agar and 70 min with human feces from the beginning of DNA extraction to final determination.
conclusionThe LAMP assay is a sensitive, rapid and simple tool for the detection of CT-producing V. cholerae and will be useful in facilitating the early diagnosis of human V. cholerae infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.