ArticleThe Plant journal : for cell and molecular biology2008
Molecular cloning and characterization of OsCDase, a ceramidase enzyme from rice.
Article in The Plant journal : for cell and molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Sphingolipids at Plasmodesmata: Structural Components and Functional Modulators.International journal of molecular sciences · 2022Review
- Sphingolipid metabolism, transport, and functions in plants: Recent progress and future perspectives.Plant communications · 2021Review
- A potential pathway for flippase-facilitated glucosylceramide catabolism in plants.Plant signaling & behavior · 2020Article
- Sphingolipids: towards an integrated view of metabolism during the plant stress response.The New phytologist · 2020Review
- A neutral ceramidase, NlnCDase, is involved in the stress responses of brown planthopper, Nilaparvata lugens (Stål).Scientific reports · 2018Article
- Article
- Plant sphingolipids: Their importance in cellular organization and adaption.Biochimica et biophysica acta · 2016Review
- The Arabidopsis alkaline ceramidase TOD1 is a key turgor pressure regulator in plant cells.Nature communications · 2015Article
- An Arabidopsis neutral ceramidase mutant ncer1 accumulates hydroxyceramides and is sensitive to oxidative stress.Frontiers in plant science · 2015Article
- Ethylene Modulates Sphingolipid Synthesis in Arabidopsis.Frontiers in plant science · 2015Article
- Involvement of rose aquaporin RhPIP1;1 in ethylene-regulated petal expansion through interaction with RhPIP2;1.Plant molecular biology · 2013Article
- Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase.Insect molecular biology · 2013Article
- Sphingolipids and plant defense/disease: the "death" connection and beyond.Frontiers in plant science · 2012Article
- Developmental pattern of aquaporin expression in barley (Hordeum vulgare L.) leaves.Journal of experimental botany · 2011Article
- Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum.Biochimie · 2011Article
- Drug targeting of sphingolipid metabolism: sphingomyelinases and ceramidases.British journal of pharmacology · 2011Review
- Cloning and characterization of a wheat neutral ceramidase gene Ta-CDase.Molecular biology reports · 2011Article
- A conserved cysteine motif is critical for rice ceramide kinase activity and function.PloS one · 2011Article
- Plant sphingolipids: decoding the enigma of the Sphinx.The New phytologist · 2010Review
- Very long chain fatty acid and lipid signaling in the response of plants to pathogens.Plant signaling & behavior · 2009Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
summarySphingolipids are a structurally diverse group of molecules based on long-chain sphingoid bases that are found in animal, fungal and plant cells. In contrast to the situation in animals and yeast, much less is known about the spectrum of sphingolipid species in plants and the roles they play in mediating cellular processes. Here, we report the cloning and characterization of a plant ceramidase from rice (Oryza sativa spp. Japonica cv. Nipponbare). Sequence analysis suggests that the rice ceramidase (OsCDase) is similar to mammalian neutral ceramidases. We demonstrate that OsCDase is a bona fide ceramidase by heterologous expression in the yeast double knockout mutant Deltaypc1Deltaydc1 that lacks the yeast ceramidases YPC1p and YDC1p. Biochemical characterization of OsCDase showed that it exhibited classical Michaelis-Menten kinetics, with optimum activity between pH 5.7 and 6.0. OsCDase activity was enhanced in the presence of Ca(2+), Mg(2+), Mn(2+) and Zn(2+), but inhibited in the presence of Fe(2+). OsCDase appears to use ceramide instead of phytoceramide as a substrate. Subcellular localization showed that OsCDase is localized to the endoplasmic reticulum and Golgi, suggesting that these organelles are sites of ceramide metabolism in plants.
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