ArticleJournal of molecular medicine (Berlin, Germany)2008
Human rheumatoid synoviocytes express functional P2X7 receptors.
Article in Journal of molecular medicine (Berlin, Germany), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- P2X7R of synovial fibroblasts is a potential therapeutic target associated with refractory rheumatoid arthritis.Science advances · 2026Article
- A Hybrid Approach Combining Shape-Based and Docking Methods to Identify Novel Potential P2X7 Antagonists from Natural Product Databases.Pharmaceuticals (Basel, Switzerland) · 2024Article
- The P2X7 Receptor in Autoimmunity.International journal of molecular sciences · 2023Review
- Article
- An overview of carbonic anhydrases and membrane channels of synoviocytes in inflamed joints.Journal of enzyme inhibition and medicinal chemistry · 2019Review
- Review
- The Purinergic System as a Pharmacological Target for the Treatment of Immune-Mediated Inflammatory Diseases.Pharmacological reviews · 2019Review
- Signaling of the Purinergic System in the Joint.Frontiers in pharmacology · 2019Review
- [Diagnostic value of P2X7 receptor and its role in inflammatory reaction in rheumatoid arthritis].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Anti-inflammatory Effect of Somatostatin Analogue Octreotide on Rheumatoid Arthritis Synoviocytes.Inflammation · 2018Article
- A cyclic pathway of P2 × 7, bradykinin, and dopamine receptor activation induces a sustained articular hyperalgesia in the knee joint of rats.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2018Article
- Updating osteoimmunology: regulation of bone cells by innate and adaptive immunity.Nature reviews. Rheumatology · 2018Review
- Immunometabolism in early and late stages of rheumatoid arthritis.Nature reviews. Rheumatology · 2017Review
- Searching Novel Therapeutic Targets for Scleroderma: P2X7-Receptor Is Up-regulated and Promotes a Fibrogenic Phenotype in Systemic Sclerosis Fibroblasts.Frontiers in pharmacology · 2017Article
- A critical look at the function of the P2Y11 receptor.Purinergic signalling · 2016Review
- Spotlight on sirukumab for the treatment of rheumatoid arthritis: the evidence to date.Drug design, development and therapy · 2016Review
- The role of P2X7 receptors in tissue fibrosis: a brief review.Purinergic signalling · 2015Review
- Molecular imaging of rheumatoid arthritis: emerging markers, tools, and techniques.Arthritis research & therapy · 2014Review
- The role of nucleotides and purinergic signaling in apoptotic cell clearance - implications for chronic inflammatory diseases.Frontiers in immunology · 2014Review
- Purinergic signalling in the musculoskeletal system.Purinergic signalling · 2013Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human type B synoviocytes are involved in joint injury during rheumatic diseases by producing inflammatory mediators such as interleukin-6 (IL-6). The increased level of purine and pirimidine nucleotides in the synovial fluid of rheumatoid arthritis (RA) patients could activate the large family of P2 receptors. Thus, we investigated the presence of P2 receptors in human type B synoviocytes from rheumatoid joints, also evaluating whether the P2X7 receptor is involved in IL-6 release. Reverse transcriptase polymerase chain reaction analysis revealed messenger ribonucleic acid (mRNA) expression for the P2X1, P2X2, P2X4, P2X5, P2X6, P2X7, P2Y1, P2Y4, P2Y11, P2Y12, P2Y13, and P2Y14 but not the P2X3, P2Y2, and P2Y6 receptors. The expression of the P2X7 receptor was confirmed by Western blot analysis. Adenosine triphosphate (ATP) and the P2X7 receptor agonist 2'-3'-O-(4-benzoylbenzoyl)ATP (BzATP) triggered an increase in intracellular calcium, thereby suggesting the expression of functional P2 receptors, including the P2X7 receptor. Moreover, BzATP treatment upregulated both IL-6 mRNA and protein expression. Synoviocytes spontaneously released low quantities of IL-6; the incubation with BzATP induced the release of larger amounts of the cytokine, and such a release was blunted by the P2X7 antagonist oxidized ATP. The selective P2X1 and P2X3 receptor agonist alpha,beta-methylene ATP did not affect IL-6 release. Finally, BzATP failed to induce a significant uptake of the large-molecule YO-PRO, thus suggesting the lack of pore formation after P2X7 receptor stimulation. In conclusion, among the different P2 receptors expressed on human RA type B synoviocytes, the P2X7 receptor may modulate IL-6 release but not inducing changes in cell membrane permeability.
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