ArticlePloS one2008
Cohesin interaction with centromeric minichromosomes shows a multi-complex rod-shaped structure.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- It's all in the numbers: Cohesin stoichiometry.Frontiers in molecular biosciences · 2022Review
- A second Wpl1 anti-cohesion pathway requires dephosphorylation of fission yeast kleisin Rad21 by PP4.The EMBO journal · 2017Article
- Of Rings and Rods: Regulating Cohesin Entrapment of DNA to Generate Intra- and Intermolecular Tethers.PLoS genetics · 2016Review
- The spatial segregation of pericentric cohesin and condensin in the mitotic spindle.Molecular biology of the cell · 2013Article
- Individual pericentromeres display coordinated motion and stretching in the yeast spindle.The Journal of cell biology · 2013Article
- Sister chromatid cohesion establishment occurs in concert with lagging strand synthesis.Cell cycle (Georgetown, Tex.) · 2012Article
- Cohesin proteins load sequentially during prophase I in tomato primary microsporocytes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Epitope tag-induced synthetic lethality between cohesin subunits and Ctf7/Eco1 acetyltransferase.FEBS letters · 2010Article
- Centromere identity, function, and epigenetic propagation across cell divisions.Cold Spring Harbor symposia on quantitative biology · 2010Article
- Yeast cohesin complex embraces 2 micron plasmid sisters in a tri-linked catenane complex.Nucleic acids research · 2010Article
- Establishment of sister chromatid cohesion.Current biology : CB · 2009Review
- Condensin: Architect of mitotic chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Review
- Article
- A handcuff model for the cohesin complex.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cohesin is the protein complex responsible for maintaining sister chromatid cohesion. Cohesin interacts with centromeres and specific loci along chromosome arms known as Chromosome Attachment Regions (CARs). The cohesin holocomplex contains four subunits. Two of them, Smc1p (Structural maintenance of chromosome 1 protein) and Smc3p, are long coiled-coil proteins, which heterodimerize with each other at one end. They are joined together at the other end by a third subunit, Scc1p, which also binds to the fourth subunit, Scc3p. How cohesin interacts with chromosomes is not known, although several models have been proposed, in part on the basis of in vitro assembly of purified cohesin proteins. To be able to observe in vivo cohesin-chromatin interactions, we have modified a Minichromosome Affinity Purification (MAP) method to isolate a CAR-containing centromeric minichromosome attached to in vivo assembled cohesin. Transmission Electron Microscopy (TEM) analysis of these minichromosomes suggests that cohesin assumes a rod shape and interacts with replicated minichromosome at one end of that rod. Additionally, our data implies that more than one cohesin molecule interacts with each pair of replicated minichromsomes. These molecules seem to be packed into a single thick rod, suggesting that the Smc1p and Smc3p subunits may interact extensively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.