ArticleBiochemistry2008
Catalytic diversity of extended hammerhead ribozymes.
Article in Biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Enhanced hammerhead ribozyme turnover rates: Reevaluating therapeutic space for small catalytic RNAs.Molecular therapy. Nucleic acids · 2025Article
- In vitro ribosome synthesis and evolution through ribosome display.Nature communications · 2020Article
- Systematic Screening, Rational Development, and Initial Optimization of Efficacious RNA Silencing Agents for Human Rod Opsin Therapeutics.Translational vision science & technology · 2019Article
- Comparative Identification of MicroRNAs inInsects · 2019Article
- Identification and profiling of Cyprinus carpio microRNAs during ovary differentiation by deep sequencing.BMC genomics · 2017Article
- The Hammerhead Ribozyme: A Long History for a Short RNA.Molecules (Basel, Switzerland) · 2017Review
- Ligation of RNA Oligomers by the Schistosoma mansoni Hammerhead Ribozyme in Frozen Solution.Journal of molecular evolution · 2016Article
- Identification and Characterization of Sex-Biased MicroRNAs in Bactrocera dorsalis (Hendel).PloS one · 2016Article
- MicroRNA-Sequence Profiling Reveals Novel Osmoregulatory MicroRNA Expression Patterns in Catadromous Eel Anguilla marmorata.PloS one · 2015Article
- Protein-responsive ribozyme switches in eukaryotic cells.Nucleic acids research · 2014Article
- The impact of transcriptional tuning on in vitro integrated rRNA transcription and ribosome construction.Nucleic acids research · 2014Article
- G17-modified hammerhead ribozymes are active in vitro and in vivo.RNA (New York, N.Y.) · 2013Article
- Metal ion as both a cofactor and a probe of metal-binding sites in a uranyl-specific DNAzyme: a uranyl photocleavage study.Nucleic acids research · 2013Article
- Bridging the gap between theory and experiment to derive a detailed understanding of hammerhead ribozyme catalysis.Progress in molecular biology and translational science · 2013Review
- Identification and characterization of microRNA in the dairy goat (Capra hircus) mammary gland by Solexa deep-sequencing technology.Molecular biology reports · 2012Article
- The ubiquitous hammerhead ribozyme.RNA (New York, N.Y.) · 2012Review
- Structure-function analysis from the outside in: long-range tertiary contacts in RNA exhibit distinct catalytic roles.Biochemistry · 2011Article
- Structure-based search reveals hammerhead ribozymes in the human microbiome.The Journal of biological chemistry · 2011Article
- Long-range tertiary interactions in single hammerhead ribozymes bias motional sampling toward catalytically active conformations.RNA (New York, N.Y.) · 2010Article
- Enhanced product stability in the hammerhead ribozyme.Biochemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chimeras of the well-characterized minimal hammerhead 16 and nine extended hammerheads derived from natural viroids and satellite RNAs were constructed with the goal of assessing whether their very different peripheral tertiary interactions modulate their catalytic properties. For each chimera, three different assays were used to determine the rate of cleavage and the fraction of full-length hammerhead at equilibrium and thereby deduce the elemental cleavage ( k 2) and ligation ( k -2) rate constants. The nine chimeras were all more active than minimal hammerheads and exhibited a very broad range of catalytic properties, with values of k 2 varying by 750-fold and k -2 by 100-fold. At least two of the hammerheads exhibited an altered dependence of k obs on magnesium concentration. Since much less catalytic diversity is observed among minimal hammerheads that lack the tertiary interactions, a possible role for the different tertiary interaction is to modulate the hammerhead cleavage properties in viroids. For example, differing hammerhead cleavage and ligation rates could affect the steady state concentrations of linear, circular, and polymeric genomes in infected cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.