ArticleMolecular and cellular biochemistry2008
Expression of TGH and its role in porcine primary adipocyte lipolysis.
Article in Molecular and cellular biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Fine-tuned iron availability is essential to achieve optimal adipocyte differentiation and mitochondrial biogenesis.Diabetologia · 2014Article
- Mice lacking G0S2 are lean and cold-tolerant.Cancer biology & therapy · 2014Article
- Mammalian triacylglycerol metabolism: synthesis, lipolysis, and signaling.Chemical reviews · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTriacylglycerol hydrolase (TGH) plays an important role in intracellular lipid metabolism. However, most previous studies on TGH were focused on rodent animals. Pig is considered as one of the best models for studying obesity, diabetes, and other lipid metabolism related diseases. So far, we do not know whether TGH is to play a role in lipolysis in porcine primary adipocyte through stimulation by hormones as Hormone-sensitive lipase (HSL). The objective of this study is to explore the mechanisms that regulate TGH expression in porcine adipocyte and its role in fasting-induced and Isoproterenol-stimulated lipolysis in porcine primary adipocytes. MATERIALS AND
methodsStromal-vascular cells containing preadipocytes were isolated from cervical and dorsal subcutaneous adipose tissues of approximately 3-day-old Chinese male piglets. After confluence, the differentiation was induced by Insulin, hydrocortisone, and transferrin. The primary adipocytes were cultured in various concentration (0.1 to 200 micromol/l) Isoproterenol for 0-12 h or serum-free medium after differentiation. The glycerol release and triacylglycerol (TG) contents were detected when the cells were collected. Then, expression of TGH and HSL mRNA and their protein were determined by RT-PCR, Western Blot, and lipolytic analysis.
resultsBoth TGH mRNA and protein were not detected on day 0, but as differentiation was induced, their expression displayed a great increase. The expression of TGH mRNA showed the highest level on day 4, but its protein reached the highest level on day 6 and began to fall down from day 8. The expression of TGH mRNA and proteins was increased in serum-free media, and mRNA expression was decreased while changed into complete media again. The glycerol release increased significantly when the cells were cultured with serum-free media. The lower concentration of Isoproterenol (0.1 and 1 micromol/l) did not affect the expression of TGH and HSL, but higher concentration (10, 100, and 200 micromol/l) could greatly up-regulate HSL expression but did not affect TGH level. Also, the higher concentration of Isoproterenol could increase the glycerol release and decrease TG content in dose-dependent manner.
conclusionsThese results suggested that TGH expression is differentiation-dependent in porcine primary adipocytes and TGH plays a role in fasting-induced lipolysis not hormone-stimulated lipolysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.