ArticleClinical & experimental metastasis2008
Metastatic breast cancer cells colonize and degrade three-dimensional osteoblastic tissue in vitro.
Article in Clinical & experimental metastasis, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- A systematic review on in vitro 3D bone metastases models: A new horizon to recapitulate the native clinical scenario?Oncotarget · 2016Pooled it
- Exploring bone-tumor interactions through 3DJournal of bone oncology · 2025Review
- Development of an alginate-Matrigel hydrogel system to evaluate cancer cell behavior in the stiffness range of the bone marrow.Frontiers in biomaterials science · 2023Article
- The Role of Inflammation in Breast and Prostate Cancer Metastasis to Bone.International journal of molecular sciences · 2021Review
- Review
- Mechanobiological evaluation of prostate cancer metastasis to bone using an in vitro prostate cancer testbed.Journal of biomechanics · 2021Article
- Trends in Bone Metastasis Modeling.Cancers · 2020Review
- What Is the Role of Interleukins in Breast Cancer Bone Metastases? A Systematic Review of Preclinical and Clinical Evidence.Cancers · 2019Review
- In Vitro 3D Cultures to Reproduce the Bone Marrow Niche.JBMR plus · 2019Review
- Mechanical and Mechanosensing Properties of Tumor Affected Bone Cells Were Inhibited via PI3K/Akt Pathway.Journal of bone metabolism · 2019Article
- 3D models of the bone marrow in health and disease: yesterday, today and tomorrow.MRS communications · 2019Article
- A Three-Dimensional Dense Collagen Hydrogel to Model Cancer Cell/Osteoblast Interactions.Journal of functional biomaterials · 2018Article
- Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis.Bone research · 2018Review
- Three-Dimensional Mechanical Loading Modulates the Osteogenic Response of Mesenchymal Stem Cells to Tumor-Derived Soluble Signals.Tissue engineering. Part A · 2016Article
- Methods for culturing human femur tissue explants to study breast cancer cell colonization of the metastatic niche.Journal of visualized experiments : JoVE · 2015Article
- Three-dimensional in vitro tumor models for cancer research and drug evaluation.Biotechnology advances · 2014Review
- The role of engineering approaches in analysing cancer invasion and metastasis.Nature reviews. Cancer · 2013Review
- Location matters: osteoblast and osteoclast distribution is modified by the presence and proximity to breast cancer cells in vivo.Clinical & experimental metastasis · 2012Article
- Global gene expression analysis of the interaction between cancer cells and osteoblasts to predict bone metastasis in breast cancer.PloS one · 2012Article
- Osteogenesis in vitro: from pre-osteoblasts to osteocytes: a contribution from the Osteobiology Research Group, The Pennsylvania State University.In vitro cellular & developmental biology. Animal · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metastatic breast cancer cells (BCs) colonize a mineralized three-dimensional (3D) osteoblastic tissue (OT) grown from isolated pre-osteoblasts for up to 5 months in a specialized bioreactor. Sequential stages of BC interaction with OT include BC adhesion, penetration, colony formation, and OT reorganization into "Indian files" paralleling BC colonies, heretofore observed only in authentic pathological cancer tissue. BCs permeabilize OT by degrading the extra-cellular collagenous matrix (ECM) in which the osteoblasts are embedded. OT maturity (characterized by culture age and cell phenotype) profoundly affects the patterns of BC colonization. BCs rapidly form colonies on immature OT (higher cell/ECM ratio, osteoblastic phenotype) but fail to completely penetrate OT. By contrast, BCs efficiently penetrate mature OT (lower cell/ECM ratio, osteocytic phenotype) and reorganize OT. BC colonization provokes a strong osteoblast inflammatory response marked by increased expression of the pro-inflammatory cytokine IL-6. Furthermore, BCs inhibit osteoblastic bone formation by down-regulating synthesis of collagen and osteocalcin. Results strongly suggest that breast cancer disrupts the process of osteoblastic bone formation, in addition to upregulating osteoclastic bone resorption as widely reported. These observations may help explain why administration of bisphosphonates to humans with osteolytic metastases slows lesion progression by inhibiting osteoclasts but does not bring about osteoblast-mediated healing.
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