ArticlePlant cell reports2008
An intensive understanding of vacuum infiltration transformation of pakchoi (Brassica rapa ssp. chinensis).
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.The Plant cell · 2025Review
- Review
- BrKCS6 mutation conferred a bright glossy phenotype to Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Optimization of Regeneration andPlants (Basel, Switzerland) · 2022Article
- Ectopic expression of nucleolar DEAD-Box RNA helicase OsTOGR1 confers improved heat stress tolerance in transgenic Chinese cabbage.Plant cell reports · 2020Article
- Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.BMC plant biology · 2019Article
- Transgenic Wucai (Brassica campestris L.) produced via Agrobacterium-mediated anther transformation in planta.Plant cell reports · 2019Article
- A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B.Plant physiology · 2017Article
- Pollen grains as a target for introduction of foreign genes into plants: an assessment.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- Analysis of Brassica rapa ESTs: gene discovery and expression patterns of AP2/ERF family genes.Molecular biology reports · 2010Article
- Robust circadian rhythms of gene expression in Brassica rapa tissue culture.Plant physiology · 2010Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pakchoi (Brassica rapa L. ssp. chinensis), a kind of Chinese cabbage, is an important vegetable in Asian countries. Agrobacterium mediated in planta vacuum infiltration transformation has been performed in pakchoi since 1998, but a detailed study on this technique was lacking. Pakchoi plants 40-50 days old with inflorescences were vacuum infiltrated with Agrobacterium tumefaciens strain C58C1 harboring the binary vector pBBBast-gus-intron. The transformation frequency in the harvested seeds mainly varied from 1 x 10(-4) to 3 x 10(-4) over several years, and it was lower than the frequency in Arabidopsis thaliana. Transformants were obtained from both the upper and the lower parts of the infiltrated plants with or without an elongated inflorescence. Stained ovules and pollen grains were found in the unopened flower 13 days post-infiltration, which was about 0.5-1 mm in diameter at infiltration time with an open ovary as revealed by paraffin sections. Histochemical assays revealed that Agrobacteria were more abundant in the flower tissue than in stem and leaf tissues at all times after infiltration despite the sharp decrease of live Agrobacteria in plant 14 days post infiltration as revealed by the colony forming units on the Agrobacteria culture medium. The results of vacuum infiltration transformation of pakchoi and Arabidopsis thaliana were compared and a strategy to optimize the transformation conditions to increase the transformation frequency in pakchoi was discussed.
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