ArticleAnalytical biochemistry2008
Real-time PCR detection of protein analytes with conformation-switching aptamers.
Article in Analytical biochemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 46 citations in OpenAlex.
- Assay methods based on proximity-enhanced reactions for detecting non-nucleic acid molecules.Frontiers in bioengineering and biotechnology · 2023Review
- Test Strip Platform Spin-Off for Telomerase Activity Detection: Development of an Electrochemical Biosensor.ACS omega · 2022Article
- Detection of ochratoxin A by aptamer-assisted real-time PCR-based assay (Apta-qPCR).Engineering in life sciences · 2017Article
- Proximity assays for sensitive quantification of proteins.Biomolecular detection and quantification · 2015Review
- Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell-free protein synthesis.ACS synthetic biology · 2014Article
- Responsive DNA-based hydrogels and their applications.Macromolecular rapid communications · 2013Review
- Nucleic acids for ultra-sensitive protein detection.Sensors (Basel, Switzerland) · 2013Review
- Diffractometric detection of proteins using microbead-based rolling circle amplification.Analytical chemistry · 2010Article
- Aptamer nano-flares for molecular detection in living cells.Nano letters · 2009Article
- Application and analysis of structure-switching aptamers for small molecule quantification.Analytica chimica acta · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a novel method that uses conformation-switching aptamers for real-time PCR analysis of protein analytes. The aptamers have been designed so that they assume one secondary structure in the absence of a protein analyte and a different secondary structure in the presence of a protein such as thrombin or platelet-derived growth factor (PDGF). The protein-bound structure in turn assembles a ligation junction for the addition of a real-time PCR primer. Protein concentrations could be specifically detected into the picomolar range, even in the presence of cell lysates. The method has advantages relative to both immunoPCR (because no signal is produced by background binding) and the proximity ligation assay (PLA) (because only one epitope, rather than two epitopes, on a protein surface must be bound).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.