ArticleApplied and environmental microbiology2008
Protein kinase A regulates growth, sporulation, and pigment formation in Aspergillus fumigatus.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 85 citations in OpenAlex.
- Multi-omics analysis reveals Protein Kinase A-associated regulatory remodeling during adaptation of Trichoderma reesei to lignocellulosic substrate.World journal of microbiology & biotechnology · 2026Article
- Properties of Putative APSES Transcription Factor AfpA inJournal of fungi (Basel, Switzerland) · 2025Article
- Development and optimization of large-scale approaches to identify iron-related genes inFrontiers in microbiology · 2025Article
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- Post-translational modifications drive secondary metabolite biosynthesis in Aspergillus: a review.Environmental microbiology · 2022Review
- Comprehensively dissecting the hub regulation of PkaC on high-productivity and pellet macromorphology in citric acid producing Aspergillus niger.Microbial biotechnology · 2022Article
- Discovery of fungal surface NADases predominantly present in pathogenic species.Nature communications · 2021Article
- Article
- The Putative APSES Transcription Factor RgdA Governs Growth, Development, Toxigenesis, and Virulence in Aspergillus fumigatus.mSphere · 2020Article
- Article
- Article
- Sporulation inJournal of fungi (Basel, Switzerland) · 2020Review
- Phosphoproteomics of Aspergillus fumigatus Exposed to the Antifungal Drug Caspofungin.mSphere · 2020Article
- Article
- RgsD negatively controls development, toxigenesis, stress response, and virulence in Aspergillus fumigatus.Scientific reports · 2019Article
- The role of PKAc1 in gene regulation and trichodimerol production inFungal biology and biotechnology · 2019Article
- The Yin and Yang of Current Antifungal Therapeutic Strategies: How Can We Harness Our Natural Defenses?Frontiers in pharmacology · 2019Review
- Article
- The APSES GeneFrontiers in microbiology · 2018Article
- Developmental regulators in Aspergillus fumigatus.Journal of microbiology (Seoul, Korea) · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspergillus fumigatus is an opportunistic human pathogenic fungus causing severe infections in immunocompromised patients. Cyclic AMP (cAMP) signal transduction plays an important role in virulence. A central component of this signaling cascade is protein kinase A (PKA), which regulates cellular processes by phosphorylation of specific target proteins. Here we describe the generation and analysis of A. fumigatus mutants expressing the gene encoding the catalytic subunit of PKA, pkaC1, under control of an inducible promoter. Strains overexpressing pkaC1 showed high PKA activity, reduced growth, sporulation deficiency, and formation of a dark pigment in the mycelium. These data indicate that cAMP-PKA signaling is involved in the regulation of important processes, such as growth, asexual reproduction, and biosynthesis of secondary metabolites. Furthermore, elevated PKA activity led to increased expression of the pksP gene. The polyketide synthase PksP is an essential enzyme for production of dihydroxynaphthalene-melanin in A. fumigatus and contributes to virulence. Our results suggest that increased pksP expression is responsible for pigment formation in the mycelium. Comparative proteome analysis of the pkaC1-overexpressing strain and the wild-type strain led to the identification of proteins regulated by the cAMP-PKA signal transduction pathway. We showed that elevated PKA activity resulted in activation of stress-associated proteins and of enzymes involved in protein biosynthesis and glucose catabolism. In contrast, proteins which were involved in nucleotide and amino acid biosynthesis were downregulated, as were enzymes involved in catabolism of carbon sources other than glucose.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.