ArticleApplied and environmental microbiology2008
Expression of green fluorescent protein fused to magnetosome proteins in microaerophilic magnetotactic bacteria.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 100 citations in OpenAlex.
- A cumate-inducible promoter system for tuned gene expression and magnetosome overproduction in magnetotactic bacteria.Applied microbiology and biotechnology · 2026Article
- The Role of Confinement in Biomineralization.Chemical reviews · 2025Review
- Cellular Distribution and Motion of Essential Magnetosome Proteins Expressed in Mammalian Cells.Biosensors · 2025Article
- Magnetizing Biotech-Advances in (In Vivo) Magnetic Enzyme Immobilization.Engineering in life sciences · 2025Review
- Amended Ferrozine Assay for Quantifying Magnetosome Iron Content in Magnetotactic Bacteria.ACS omega · 2024Article
- Understanding Biomineralization Mechanisms to Produce Size-Controlled, Tailored Nanocrystals for Optoelectronic and Catalytic Applications: A Review.ACS applied nano materials · 2024Review
- Intrinsic and extrinsic determinants of conditional localization of Mms6 to magnetosome organelles inJournal of bacteriology · 2024Article
- Biosynthesis of magnetosome-nanobody complex in Magnetospirillum gryphiswaldense MSR-1 and a magnetosome-nanobody-based enzyme-linked immunosorbent assay for the detection of tetrabromobisphenol A in water.Analytical and bioanalytical chemistry · 2024Article
- Biomedical applications of magnetosomes: State of the art and perspectives.Bioactive materials · 2023Review
- Comparing the Colloidal Stabilities of Commercial and Biogenic Iron Oxide Nanoparticles That Have Potential In Vitro/In Vivo Applications.Molecules (Basel, Switzerland) · 2023Article
- Emerging enzyme surface display systems for waste resource recovery.Environmental microbiology · 2023Review
- Live-Cell Fluorescence Imaging of Magnetosome Organelle for Magnetotaxis Motility.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Magnetosome-Based Platform for Flow Biocatalysis.ACS applied materials & interfaces · 2022Article
- Biocompatibility, uptake and subcellular localization of bacterial magnetosomes in mammalian cells.Nanoscale advances · 2021Article
- Tumor-targeting inorganic nanomaterials synthesized by living cells.Nanoscale advances · 2021Review
- Induction of Axonal Outgrowth in Mouse Hippocampal Neurons via Bacterial Magnetosomes.International journal of molecular sciences · 2021Article
- Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation.Frontiers in bioengineering and biotechnology · 2020Review
- MamY is a membrane-bound protein that aligns magnetosomes and the motility axis of helical magnetotactic bacteria.Nature microbiology · 2019Article
- Flow cytometry as a rapid analytical tool to determine physiological responses to changing OScientific reports · 2017Article
- Segregation of prokaryotic magnetosomes organelles is driven by treadmilling of a dynamic actin-like MamK filament.BMC biology · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The magnetosomes of magnetotactic bacteria are prokaryotic organelles consisting of a magnetite crystal bounded by a phospholipid bilayer that contains a distinct set of proteins with various functions. Because of their unique magnetic and crystalline properties, magnetosome particles are potentially useful as magnetic nanoparticles in a number of applications, which in many cases requires the coupling of functional moieties to the magnetosome membrane. In this work, we studied the use of green fluorescent protein (GFP) as a reporter for the magnetosomal localization and expression of fusion proteins in the microaerophilic Magnetospirillum gryphiswaldense by flow cytometry, fluorescence microscopy, and biochemical analysis. Although optimum conditions for high fluorescence and magnetite synthesis were mutually exclusive, we established oxygen-limited growth conditions, which supported growth, magnetite biomineralization, and GFP fluorophore formation at reasonable rates. Under these optimized conditions, we studied the subcellular localization and expression of the GFP-tagged magnetosome proteins MamC, MamF, and MamG by fluorescence microscopy and immunoblotting. While all fusions specifically localized at the magnetosome membrane, MamC-GFP displayed the strongest expression and fluorescence. MamC-GFP-tagged magnetosomes purified from cells displayed strong fluorescence, which was sensitive to detergents but stable under a wide range of temperature and salt concentrations. In summary, our data demonstrate the use of GFP as a reporter for protein localization under magnetite-forming conditions and the utility of MamC as an anchor for magnetosome-specific display of heterologous gene fusions.
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