ArticleApplied and environmental microbiology2008
Genome sequence and characteristics of Lrm1, a prophage from industrial Lactobacillus rhamnosus strain M1.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
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- Occurrence and genetic diversity of prophage sequences identified in the genomes of L. casei group bacteria.Scientific reports · 2023Article
- The enemy from within: a prophage of Roseburia intestinalis systematically turns lytic in the mouse gut, driving bacterial adaptation by CRISPR spacer acquisition.The ISME journal · 2020Article
- Lumen and mucosa-associatedGut microbiome (Cambridge, England) · 2020Article
- Genomic and Proteomic Characterization of Bacteriophage BH1 Spontaneously Released from ProbioticViruses · 2019Article
- Complete genome sequence ofGut pathogens · 2018Article
- The Variable Regions of Lactobacillus rhamnosus Genomes Reveal the Dynamic Evolution of Metabolic and Host-Adaptation Repertoires.Genome biology and evolution · 2016Article
- A novel chimeric prophage vB_LdeS-phiJB from commercial Lactobacillus delbrueckii subsp. bulgaricus.Journal of industrial microbiology & biotechnology · 2016Article
- Genomic Diversity of Phages Infecting Probiotic Strains of Lactobacillus paracasei.Applied and environmental microbiology · 2016Article
- Exposing the secrets of two well-known Lactobacillus casei phages, J-1 and PL-1, by genomic and structural analysis.Applied and environmental microbiology · 2014Article
- The genome of the Lactobacillus sanfranciscensis temperate phage EV3.BMC research notes · 2013Article
- Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease.Nucleic acids research · 2013Article
- Characterization of two virulent phages of Lactobacillus plantarum.Applied and environmental microbiology · 2012Article
- Bacteriophages and dairy fermentations.Bacteriophage · 2012Article
- Bacteriophages of lactic acid bacteria and their impact on milk fermentations.Microbial cell factories · 2011Review
- Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human- mucus binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prophage Lrm1 was induced with mitomycin C from an industrial Lactobacillus rhamnosus starter culture, M1. Electron microscopy of the lysate revealed relatively few intact bacteriophage particles among empty heads and disassociated tails. The defective Siphoviridae phage had an isometric head of approximately 55 nm and noncontractile tail of about 275 nm with a small baseplate. In repeated attempts, the prophage could not be cured from L. rhamnosus M1, nor could a sensitive host be identified. Sequencing of the phage Lrm1 DNA revealed a genome of 39,989 bp and a G+C content of 45.5%. A similar genomic organization and mosaic pattern of identities align Lrm1 among the closely related Lactobacillus casei temperate phages A2, PhiAT3, and LcaI and with L. rhamnosus virulent phage Lu-Nu. Of the 54 open reading frames (ORFs) identified, all but 8 shared homology with other phages of this group. Five unknown ORFs were identified that had no homologies in the databases nor predicted functions. Notably, Lrm1 encodes a putative endonuclease and a putative DNA methylase with homology to a methylase in Lactococcus lactis phage Tuc2009. Possibly, the DNA methylase, endonuclease, or other Lrm1 genes provide a function crucial to L. rhamnosus M1 survival, resulting in the stability of the defective prophage in its lysogenic state. The presence of a defective prophage in an industrial strain could provide superinfection immunity to the host but could also contribute DNA in recombination events to produce new phages potentially infective for the host strain in a large-scale fermentation environment.
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