ArticleApplied and environmental microbiology2008
Implication of an outer surface lipoprotein in adhesion of Bifidobacterium bifidum to Caco-2 cells.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 121 citations in OpenAlex.
- Consumption of a Bifidobacterium bifidum Strain for 4 Weeks Modulates Dominant Intestinal Bacterial Taxa and Fecal Butyrate in Healthy Adults.Applied and environmental microbiology · 2016Trial
- Integrated Phenotypic and Genomic Characterization Reveals Functional Heterogeneity among Four Bifidobacterium Strains.Probiotics and antimicrobial proteins · 2026Article
- Bifidobacteria: biogeography, host adaptation and ecological functions.Nature reviews. Microbiology · 2026Review
- Mucin O-glycan degradation by GH101 underpins mucosal persistence of Bifidobacterium bifidum PRL2010.Applied microbiology and biotechnology · 2026Article
- Comprehensive Evaluation of Safety, Functional Properties, and Immunomodulatory Activity of 19 Probiotic Strains Approved by the Ministry of Food and Drug Safety (MFDS).Journal of microbiology and biotechnology · 2026Article
- Viable and Heat-InactivatedMicroorganisms · 2026Article
- Host interactions ofApplied and environmental microbiology · 2025Article
- Heyndrickxia coagulans LMG S-24828 Is a Safe Probiotic Strain Capable of Germinating in the Human Gut.Probiotics and antimicrobial proteins · 2025Article
- Probiotic Bifidobacterium bifidum strains desialylate MUC13 and increase intestinal epithelial barrier function.Scientific reports · 2025Article
- The role of teichoic acids of bifidobacteria in driving the interaction with the human host.Frontiers in microbiology · 2025Article
- Understanding the interaction between melatonin and bifidobacteria.Microbiome research reports · 2025Article
- Characterization of aApplied and environmental microbiology · 2024Article
- From raw milk cheese to the gut: investigating the colonization strategies ofApplied and environmental microbiology · 2024Article
- Ecology- and genome-based identification of theApplied and environmental microbiology · 2024Article
- GH136-encoding gene (perB) is involved in gut colonization and persistence by Bifidobacterium bifidum PRL2010.Microbial biotechnology · 2024Article
- Genomic and ecological approaches to identify theFrontiers in microbiology · 2024Article
- Exploring Molecular Interactions between Human Milk Hormone Insulin and Bifidobacteria.Microbiology spectrum · 2023Article
- HMO-primed bifidobacteria exhibit enhanced ability to adhere to intestinal epithelial cells.Frontiers in microbiology · 2023Article
- Native and Engineered Probiotics: Promising Agents against Related Systemic and Intestinal Diseases.International journal of molecular sciences · 2022Review
- Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we labeled BopA. We purified the protein chromatographically and found that it functioned as an adhesion promoter on Caco-2 cells. In silico analysis of the gene coding for this protein and globomycin experiments showed that BopA is a cysteine-anchored lipoprotein expressed as a precursor polypeptide. A database search indicated that BopA appears to function biologically as an oligopeptide/tripeptide-solute-binding protein in the ABC transport system. We discovered a protein corresponding to BopA and its gene in eight other highly adherent B. bifidum strains. Finally, we found that B. bifidum MIMBb75 and BopA affected the production of interleukin-8 in Caco-2 epithelial cells. BopA is the first protein described to date to be directly involved in the adhesion of bifidobacteria to Caco-2 cells and to show immunomodulatory activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.