ArticleJournal of bacteriology2008
Identification of a putative acetyltransferase gene, MMP0350, which affects proper assembly of both flagella and pili in the archaeon Methanococcus maripaludis.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Post-Translational Modifications Aid Archaeal Survival.Biomolecules · 2020Review
- Archaeal cell surface biogenesis.FEMS microbiology reviews · 2018Article
- Evidence that biosynthesis of the second and third sugars of the archaellin Tetrasaccharide in the archaeon Methanococcus maripaludis occurs by the same pathway used by Pseudomonas aeruginosa to make a di-N-acetylated sugar.Journal of bacteriology · 2015Article
- Pilin Processing Follows a Different Temporal Route than That of Archaellins in Methanococcus maripaludis.Life (Basel, Switzerland) · 2015Article
- Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.FEMS microbiology reviews · 2015Review
- The archaellum: how Archaea swim.Frontiers in microbiology · 2015Article
- Getting a hold on archaeal type IV pili: an expanding repertoire of cellular appendages implicates complex regulation and diverse functions.Frontiers in microbiology · 2015Article
- Archaeal type IV pili and their involvement in biofilm formation.Frontiers in microbiology · 2015Article
- Editorial.FEMS microbiology reviews · 2015Article
- N-linked glycosylation in Archaea: a structural, functional, and genetic analysis.Microbiology and molecular biology reviews : MMBR · 2014Review
- Identification of genes involved in the biosynthesis of the third and fourth sugars of the Methanococcus maripaludis archaellin N-linked tetrasaccharide.Journal of bacteriology · 2013Article
- Surface appendages of archaea: structure, function, genetics and assembly.Life (Basel, Switzerland) · 2013Review
- Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.PloS one · 2013Article
- N-glycosylation of Haloferax volcanii flagellins requires known Agl proteins and is essential for biosynthesis of stable flagella.Journal of bacteriology · 2012Article
- Identification of genes involved in the acetamidino group modification of the flagellin N-linked glycan of Methanococcus maripaludis.Journal of bacteriology · 2012Article
- Overview of the genetic tools in the Archaea.Frontiers in microbiology · 2012Article
- Diversity and subcellular distribution of archaeal secreted proteins.Frontiers in microbiology · 2012Article
- The archaeal cell envelope.Nature reviews. Microbiology · 2011Review
- Genetic and mass spectrometry analyses of the unusual type IV-like pili of the archaeon Methanococcus maripaludis.Journal of bacteriology · 2011Article
- Mass spectrometry unmasks mystery Methanococcus pilin.Journal of bacteriology · 2011Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylation is a posttranslational modification utilized in all three domains of life. Compared to eukaryotic and bacterial systems, knowledge of the archaeal processes involved in glycosylation is limited. Recently, Methanococcus voltae flagellin proteins were found to have an N-linked trisaccharide necessary for proper flagellum assembly. Current analysis by mass spectrometry of Methanococcus maripaludis flagellin proteins also indicated the attachment of an N-glycan containing acetylated sugars. To identify genes involved in sugar biosynthesis in M. maripaludis, a putative acetyltransferase was targeted for in-frame deletion. Deletion of this gene (MMP0350) resulted in a flagellin molecular mass shift to a size comparable to that expected for underglycosylated or completely nonglycoslyated flagellins, as determined by immunoblotting. Assembled flagellar filaments were not observed by electron microscopy. Interestingly, the deletion also resulted in defective pilus anchoring. Mutant cells with a deletion of MMP0350 had very few, if any, pili attached to the cell surface compared to a nonflagellated but piliated strain. However, pili were obtained from culture supernatants of this strain, indicating that the defect was not in pilus assembly but in stable attachment to the cell surface. Complementation of MMP0350 on a plasmid restored pilus attachment, but it was unable to restore flagellation, likely because the mutant ceased to make detectable flagellin. These findings represent the first report of a biosynthetic gene involved in flagellin glycosylation in archaea. Also, it is the first gene to be associated with pili, linking flagellum and pilus structure and assembly through posttranslational modifications.
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