ArticleJournal of bacteriology2008
The Bradyrhizobium japonicum Irr protein is a transcriptional repressor with high-affinity DNA-binding activity.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- In-depth transcriptomic and ChIP-seq analyses on IrrB regulation of iron homeostasis during initial life phase of Magnetospirillum gryphiswaldense.World journal of microbiology & biotechnology · 2026Article
- The divalent metal ion exporter IhpABC is required to maintain iron homeostasis under low to moderate environmental iron conditions in the bacterium Bradyrhizobium japonicum.Molecular microbiology · 2024Article
- Scanning iron response regulator binding sites using Dap-seq in the Brucella genome.PLoS neglected tropical diseases · 2023Article
- Mechanistic insights into heme-mediated transcriptional regulation via a bacterial manganese-binding iron regulator, iron response regulator (Irr).The Journal of biological chemistry · 2020Article
- Ironing Out the Unconventional Mechanisms of Iron Acquisition and Gene Regulation inFrontiers in cellular and infection microbiology · 2017Review
- A bacterial iron exporter for maintenance of iron homeostasis.The Journal of biological chemistry · 2014Article
- Differential control of Bradyrhizobium japonicum iron stimulon genes through variable affinity of the iron response regulator (Irr) for target gene promoters and selective loss of activator function.Molecular microbiology · 2014Article
- HmuP is a coactivator of Irr-dependent expression of heme utilization genes in Bradyrhizobium japonicum.Journal of bacteriology · 2012Article
- Role of the Irr protein in the regulation of iron metabolism in Rhodobacter sphaeroides.PloS one · 2012Article
- The Bradyrhizobium japonicum frcB gene encodes a diheme ferric reductase.Journal of bacteriology · 2011Article
- Peroxide stress elicits adaptive changes in bacterial metal ion homeostasis.Antioxidants & redox signaling · 2011Review
- Transcriptional control of the Bradyrhizobium japonicum irr gene requires repression by fur and Antirepression by Irr.The Journal of biological chemistry · 2010Article
- Control of bacterial iron homeostasis by manganese.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Heme-responsive DNA binding by the global iron regulator Irr from Rhizobium leguminosarum.The Journal of biological chemistry · 2010Article
- Expression of BfrH, a putative siderophore receptor of Bordetella bronchiseptica, is regulated by iron, Fur1, and the extracellular function sigma factor EcfI.Infection and immunity · 2010Article
- Coordination chemistry of bacterial metal transport and sensing.Chemical reviews · 2009Review
- Positive control of ferric siderophore receptor gene expression by the Irr protein in Bradyrhizobium japonicum.Journal of bacteriology · 2009Article
- Heme-dependent metalloregulation by the iron response regulator (Irr) protein in Rhizobium and other Alpha-proteobacteria.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2009Article
- Function, regulation, and transcriptional organization of the hemin utilization locus of Bartonella quintana.Infection and immunity · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Irr protein is a global regulator of iron homeostasis in Bradyrhizobium japonicum, and a subset of genes within the Irr regulon are negatively controlled under iron limitation. However, repressor function, high-affinity DNA binding in vitro, or promoter occupancy in vivo of Irr for a negatively regulated gene has not been demonstrated. Here, we show that the blr7895 and bll6680 genes are negatively regulated by Irr as determined by derepression of transcript levels in iron-limited cells of an irr mutant strain. Electrophoretic gel mobility shift analysis showed that a component in extracts of wild-type cells grown under iron limitation bound the iron control elements (ICE) within the promoters of blr7895 and bll6680 identified previously (G. Rudolph, G. Semini, F. Hauser, A. Lindemann, M. Friberg, H. Hennecke, and H. M. Fischer, J. Bacteriol. 188:733-744, 2006). Binding was not observed with extracts of cells from the parent strain grown under high iron conditions or with those from an irr mutant. Furthermore, gel mobility supershift experiments identified Irr as a component of the binding complex. Purified recombinant Irr bound to ICE DNA with high affinity in the presence of divalent metal, with K(d) values of 7 to 19 nM, consistent with a physiological role for Irr as a transcriptional regulator. In addition, in vitro transcription initiated from the blr7895 promoter was inhibited by Irr. Whole-cell cross-linking and immunoprecipitation experiments showed that Irr occupies the promoters of blr7895 and bll6680 in vivo in an iron-dependent manner. The findings demonstrate that Irr is a transcriptional repressor that binds DNA with high affinity.
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