ArticleMolecular cell2008
Transcription initiation in a single-subunit RNA polymerase proceeds through DNA scrunching and rotation of the N-terminal subdomains.
Article in Molecular cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 54 citations in OpenAlex.
- Process and analytical strategies for the safe production of mRNA vaccines and therapeutics.Molecular biology reports · 2026Review
- How does the polymerase of non-segmented negative strand RNA viruses commit to transcription or genome replication?Journal of virology · 2024Review
- Understanding the impact ofFrontiers in molecular biosciences · 2024Review
- Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics.Frontiers in molecular biosciences · 2024Review
- Article
- Cryo-EM Structures Reveal Transcription Initiation Steps by Yeast Mitochondrial RNA Polymerase.Molecular cell · 2021Article
- Structure, mechanism, and regulation of mitochondrial DNA transcription initiation.The Journal of biological chemistry · 2020Review
- The dynamic landscape of transcription initiation in yeast mitochondria.Nature communications · 2020Article
- The C-terminal tail of the yeast mitochondrial transcription factor Mtf1 coordinates template strand alignment, DNA scrunching and timely transition into elongation.Nucleic acids research · 2020Article
- Transcription and translation contribute to gene locus relocation to the nucleoid periphery in E. coli.Nature communications · 2019Article
- Correlating Transcription Initiation and Conformational Changes by a Single-Subunit RNA Polymerase with Near Base-Pair Resolution.Molecular cell · 2018Article
- Different types of pausing modes during transcription initiation.Transcription · 2017Article
- Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence.Protein science : a publication of the Protein Society · 2017Review
- Backtracked and paused transcription initiation intermediate of Escherichia coli RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers.Scientific reports · 2016Article
- Article
- Srs2 prevents Rad51 filament formation by repetitive motion on DNA.Nature communications · 2013Article
- Single-molecule nanometry for biological physics.Reports on progress in physics. Physical Society (Great Britain) · 2013Review
- T7 RNA polymerases backed up by covalently trapped proteins catalyze highly error prone transcription.The Journal of biological chemistry · 2012Article
- Opening-closing dynamics of the mitochondrial transcription pre-initiation complex.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Elucidating the mechanism of transcription initiation by RNA polymerases (RNAP) is essential for understanding gene transcription and regulation. Although several models, such as DNA scrunching, RNAP translation, and RNAP rotation, have been proposed, the mechanism of initiation by T7 RNAP has remained unclear. Using ensemble and single-molecule Förster resonance energy transfer (FRET) studies, we provide evidence for concerted DNA scrunching and rotation during initiation by T7 RNAP. A constant spatial distance between the upstream and downstream edges of initiation complexes making 4-7 nt RNA supports the DNA scrunching model, but not the RNAP translation or the pure rotation model. DNA scrunching is accompanied by moderate hinging motion (18 degrees +/- 4 degrees ) of the promoter toward the downstream DNA. The observed stepwise conformational changes provide a basis to understand abortive RNA synthesis during early stages of initiation and promoter escape during the later stages that allows transition to processive elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.