ArticleDiabetes care2008
A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2.
Article in Diabetes care, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Predictive Autoantibodies Before the Diagnosis of Type I Diabetes in Adults.medRxiv : the preprint server for health sciences · 2026Article
- Article
- Comparative Analysis of the Sensitivity, Specificity, Concordance, and 5-Year Predictive Power of Diabetes-Related Autoantibody Assays.Diabetes · 2025Article
- Improved Specificity of Glutamate Decarboxylase 65 Autoantibody Measurement Using Luciferase-Based Immunoprecipitation System Assays.Diabetes · 2024Article
- A novel, high-performance, low-volume, rapid luciferase immunoprecipitation system (LIPS) assay to detect autoantibodies to zinc transporter 8.Clinical and experimental immunology · 2024Article
- Tetraspanin 7 autoantibodies predict progressive decline of beta cell function in individuals with LADA.Diabetologia · 2019Article
- Autoimmune hyperphosphatemic tumoral calcinosis in a patient with FGF23 autoantibodies.The Journal of clinical investigation · 2018Article
- No evidence of pancreatic ductal adenocarcinoma specific autoantibodies to Ezrin in a liquid phase LIPS immunoassay.Cancer biomarkers : section A of Disease markers · 2018Article
- Autoantibody binding in liquid phase to IL-2 in human sera is not type 1 diabetes specific.Diabetologia · 2017Article
- Luminescent Immunoprecipitation System (LIPS) for Detection of Autoantibodies Against ATP4A and ATP4B Subunits of Gastric Proton Pump H+,K+-ATPase in Atrophic Body Gastritis Patients.Clinical and translational gastroenterology · 2017Article
- Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application.BMC biotechnology · 2016Article
- Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy.JCI insight · 2016Article
- Luciferase immunoprecipitation systems for measuring antibodies in autoimmune and infectious diseases.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Novel detection of pancreatic and duodenal homeobox 1 autoantibodies (PAA) in human sera using luciferase immunoprecipitation systems (LIPS) assay.International journal of clinical and experimental pathology · 2013Article
- Autoantibodies to harmonin and villin are diagnostic markers in children with IPEX syndrome.PloS one · 2013Article
- Extrapancreatic autoantibody profiles in type I diabetes.PloS one · 2012Article
- Searching for biomarkers: humoral response profiling with luciferase immunoprecipitation systems.Expert review of proteomics · 2011Review
- High levels of Anti-GAD65 and Anti-Ro52 autoantibodies in a patient with major depressive disorder showing psychomotor disturbance.Journal of neuroimmunology · 2010Article
- Comparison of radioimmunoprecipitation with luciferase immunoprecipitation for autoantibodies to GAD65 and IA-2beta.Diabetes care · 2010Article
- Extraordinary antigenicity of the human Ro52 autoantigen.American journal of translational research · 2010Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
objectiveInsulinoma-associated protein 2 (IA-2) is a major autoantigen in type 1 diabetes, and IA-2 autoantibodies are routinely detected by a liquid-phase radioimmunoprecipitation assay. The present experiments were initiated to develop a new assay that does not require the use of radioisotopes or autoantigens prepared in bacteria or by in vitro transcription/translation. RESEARCH DESIGN AND
methodsIA-2 luciferase fusion protein was expressed in mammalian cells and assayed for autoantibodies by liquid-phase luciferase immunoprecipitation.
resultsOur study showed that there was no significant difference between the luciferase immunoprecipitation and the radioimmunoprecipitation assays in sensitivity and specificity, and comparison of the two assays revealed a high correlation coefficient (R(2) = 0.805).
conclusionsThe luciferase system offers a robust, inexpensive, nonradioactive method for the detection of autoantibodies to mammalian cell-prepared IA-2 and could be of practical value at the clinical level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.