ArticleThe Journal of biological chemistry2008
Beta-arrestin scaffolding of phosphatidylinositol 4-phosphate 5-kinase Ialpha promotes agonist-stimulated sequestration of the beta2-adrenergic receptor.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Interaction of Angiotensin II AT1 Receptors with Purinergic P2X Receptors in Regulating Renal Afferent Arterioles in Angiotensin II-Dependent Hypertension.International journal of molecular sciences · 2023Review
- Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling.eLife · 2022Article
- Biophysical physiology of phosphoinositide rapid dynamics and regulation in living cells.The Journal of general physiology · 2022Article
- β-arrestin-dependent PI(4,5)PProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)PCell reports · 2021Article
- Metabolic Deregulation of the Blood-Outer Retinal Barrier in Retinitis Pigmentosa.Cell reports · 2019Article
- Phospholipids: Pulling Back the Actin Curtain for Granule Delivery to the Immune Synapse.Frontiers in immunology · 2019Review
- The Diverse Roles of Arrestin Scaffolds in G Protein-Coupled Receptor Signaling.Pharmacological reviews · 2017Review
- Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling.The Journal of general physiology · 2016Article
- Role of β-arrestins and arrestin domain-containing proteins in G protein-coupled receptor trafficking.Current opinion in cell biology · 2014Review
- β-arrestin promotes Wnt-induced low density lipoprotein receptor-related protein 6 (Lrp6) phosphorylation via increased membrane recruitment of Amer1 protein.The Journal of biological chemistry · 2014Article
- β-arrestins and G protein-coupled receptor trafficking.Handbook of experimental pharmacology · 2014Review
- The very large G protein coupled receptor (Vlgr1) in hair cells.Journal of molecular neuroscience : MN · 2013Review
- β-arrestin-selective G protein-coupled receptor agonists engender unique biological efficacy in vivo.Molecular endocrinology (Baltimore, Md.) · 2013Article
- β2-adrenergic receptor and astrocyte glucose metabolism.Journal of molecular neuroscience : MN · 2012Article
- Role of phosphoinositides at the neuronal synapse.Sub-cellular biochemistry · 2012Review
- Ubiquitin-mediated regulation of endocytosis by proteins of the arrestin family.Biochemistry research international · 2012Article
- β-Arrestin-mediated receptor trafficking and signal transduction.Trends in pharmacological sciences · 2011Review
- Functional relevance of biased signaling at the angiotensin II type 1 receptor.Endocrine, metabolic & immune disorders drug targets · 2011Review
- Beyond desensitization: physiological relevance of arrestin-dependent signaling.Pharmacological reviews · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Members of the seven-transmembrane receptor (7TMR) superfamily are sequestered from the plasma membrane following stimulation both to limit cellular responses as well as to initiate novel G protein-independent signaling pathways. The best studied mechanism for 7TMR internalization is via clathrin-coated pits, where clathrin and adaptor protein complex 2 nucleate and polymerize upon encountering the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) to form the outer layer of the clathrin-coated vesicle. Activated receptors are recruited to clathrin-coated pits by beta-arrestins, scaffolding proteins that interact with agonist-occupied 7TMRs as well as adaptor protein complex 2 and clathrin. We report here that following stimulation of the beta2-adrenergic receptor (beta2-AR), a prototypical 7TMR, beta-arrestins bind phosphatidylinositol 4-phosphate 5-kinase (PIP5K) Ialpha, a PIP(2)-producing enzyme. Furthermore, beta-arrestin2 is required to form a complex with PIP5K Ialpha and agonist-occupied beta2-AR, and beta-arrestins synergize with the kinase to produce PIP(2) in response to isoproterenol stimulation. Interestingly, beta-arrestins themselves bind PIP(2), and a beta-arrestin mutant deficient in PIP(2) binding no longer internalizes 7TMRs, fails to interact with PIP5K Ialpha, and is not associated with PIP kinase activity assayed in vitro. However, a chimeric protein in which the core kinase domain of PIP5K Ialpha has been fused to the same beta-arrestin mutant rescues internalization of beta2-ARs. Collectively, these data support a model in which beta-arrestins direct the localization of PIP5K Ialpha and PIP(2) production to agonist-activated 7TMRs, thereby regulating receptor internalization.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.