ArticleTraffic (Copenhagen, Denmark)2008
The prodomain of Toxoplasma gondii GPI-anchored subtilase TgSUB1 mediates its targeting to micronemes.
Article in Traffic (Copenhagen, Denmark), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- N-acetylglucosamine supplementation fails to bypass the critical acetylation of glucosamine-6-phosphate required for Toxoplasma gondii replication and invasion.PLoS pathogens · 2024Article
- Article
- A cathepsin C-like protease post-translationally modifiesbioRxiv : the preprint server for biology · 2023Article
- Do All Coccidia Follow the Same Trafficking Rules?Life (Basel, Switzerland) · 2021Review
- Directing traffic: Chaperone-mediated protein transport in malaria parasites.Cellular microbiology · 2020Review
- Article
- Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle.Scientific reports · 2017Article
- Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1.Molecular microbiology · 2017Article
- Global Analysis of Palmitoylated Proteins in Toxoplasma gondii.Cell host & microbe · 2015Article
- A HT/PEXEL motif in Toxoplasma dense granule proteins is a signal for protein cleavage but not export into the host cell.Traffic (Copenhagen, Denmark) · 2013Article
- Microneme protein 5 regulates the activity of Toxoplasma subtilisin 1 by mimicking a subtilisin prodomain.The Journal of biological chemistry · 2012Article
- Proteases as regulators of pathogenesis: examples from the Apicomplexa.Biochimica et biophysica acta · 2012Review
- Identification of Toxoplasma gondii SUB1 antigen as a marker for acute infection by use of an innovative evaluation method.Journal of clinical microbiology · 2011Article
- Forward targeting of Toxoplasma gondii proproteins to the micronemes involves conserved aliphatic amino acids.Traffic (Copenhagen, Denmark) · 2011Article
- Improved techniques for endogenous epitope tagging and gene deletion in Toxoplasma gondii.Journal of microbiological methods · 2011Article
- Toxoplasma gondii protease TgSUB1 is required for cell surface processing of micronemal adhesive complexes and efficient adhesion of tachyzoites.Cellular microbiology · 2010Article
- Coordinated progression through two subtranscriptomes underlies the tachyzoite cycle of Toxoplasma gondii.PloS one · 2010Article
- Cathepsin L occupies a vacuolar compartment and is a protein maturase within the endo/exocytic system of Toxoplasma gondii.Molecular microbiology · 2010Article
- Role of CpSUB1, a subtilisin-like protease, in Cryptosporidium parvum infection in vitro.Eukaryotic cell · 2009Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Subtilisin-like proteases have been proposed to play an important role for parasite survival in Toxoplasma gondii (Tg) and Plasmodium falciparum. The T. gondii subtilase TgSUB1 is located in the microneme, an apical secretory organelle whose contents mediate adhesion to the host during invasion. TgSUB1 is predicted to contain a glycosyl-phosphatidylinositol (GPI) anchor. This is unusual as Toxoplasma GPI-anchored proteins are targeted to the parasite's surface. In this study, we report that the subtilase TgSUB1 is indeed a GPI-anchored protein but contains dominant microneme targeting signals. Accurate targeting of TgSUB1 to the micronemes is dependent upon several factors including promoter strength and timing, accurate processing and folding. We analyzed the targeting domains of TgSUB1 using TgSUB1 deletion constructs and chimeras made between TgSUB1 and reporter proteins. The TgSUB1 prodomain is responsible for trafficking to the micronemes and is sufficient for targeting a reporter protein to the micronemes. Trafficking is dependent upon correct folding or other context-dependent conformation as the prodomain expressed alone is unable to reach the micromenes. Therefore, TgSUB1 is a novel example of a GPI-anchored protein in T. gondii that bypasses the GPI-dependent surface trafficking pathway to traffic to micronemes, specialized regulated secretory organelles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.